p53 and bcl-2 immunohistochemistry in pretreatment prostate needle biopsies to predict recurrence of prostate cancer after radical prostatectomy.
p53 and bcl-2 immunohistochemistry in pretreatment prostate needle biopsies to predict recurrence of prostate cancer after radical prostatectomy.
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p53 和 bcl-2 免疫组织化学在预处理前列腺针活检中预测根治性前列腺切除术后前列腺癌的复发。
DOI：
10.1016/s0022-5347(05)68095-0
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
J. Moul
中科院分区：
文献类型：
--
作者：
G. Stackhouse;Isabell A. Sesterhenn;J. Bauer;F. Mostofi;R. R. Connelly;S. Srivastava;J. Moul
目的：免疫组织化学染色的前列腺根治术标本中p53和bcl-2蛋白已被证明与前列腺特异性抗原（PSA）复发在我们机构的一系列患者相关。我们分析了诊断性前列腺穿刺活检中p53和bcl-2的染色与PSA复发的关系。材料与方法1986 ~ 1993年在我院行根治性前列腺切除术335例。在先前的系列中，199例前列腺切除术标本被评估为p53和bcl-2蛋白。在139例可用于分析的活检材料中，129例有足够的评估一种或两种标志物。有前瞻性的临床随访资料，平均随访6年。市售抗体用于免疫组织化学染色。结果199例根治性前列腺切除术患者PSA复发率为37.6%，129例活检免疫组化染色患者PSA复发率为37.9%。前列腺切除术染色与p53 （p = 0.004）和bcl-2 （p = 0.001）的PSA复发相关。然而，活检染色与前列腺切除术染色或PSA复发没有相关性。结论在前列腺切除术标本分析中，p53和bcl-2生物标志物对预测前列腺癌复发具有重要意义，但在前列腺活检的免疫组化染色中，这种作用并不明显。这种差异可能反映了采样误差和/或前列腺癌的异质性，值得进一步研究。
PurposeImmunohistochemical staining of radical prostatectomy specimens for p53 and bcl-2 proteins has been shown to correlate with prostate specific antigen (PSA) recurrence in a series of patients at our institution. We analyzed the relationship between staining of diagnostic prostate needle biopsies for p53 and bcl-2, and PSA recurrence.Materials and MethodsFrom 1986 to 1993, 335 radical prostatectomies were performed at our hospital. Of the prostatectomy specimens 199 had been evaluated for p53 and bcl-2 proteins in a prior series. Of 139 patients with biopsy material available for analysis 129 had enough for evaluation of 1 or both markers. Prospectively obtained clinical followup data were available, with a mean followup of 6 years. Commercially available antibodies were used for immunohistochemical staining.ResultsThe overall PSA recurrence rate was 37.6% for 199 radical prostatectomy cases and 37.9% for 129 with biopsy immunohistochemical staining. Staining of prostatectomies correlated well with PSA recurrence for p53 (p = 0.004) and bcl-2 (p = 0.001). However, biopsy staining did not correlate with prostatectomy staining or PSA recurrence for either marker.ConclusionsThe p53 and bcl-2 biomarkers appear to be important to predict recurrence of prostate cancer when prostatectomy specimens are analyzed but this usefulness is not apparent with immunohistochemical staining of prostate biopsies. This difference may reflect sampling error and/or the heterogeneous nature of prostate cancers, and deserves further study.
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人类前列腺癌中 p53 突变的肿瘤内分布的异质性。
DOI：
--
发表时间：
1995
期刊：
The American journal of pathology.
影响因子：
--
作者：
Mirchandani,D;Zheng,J;Miller,GJ;Ghosh,AK;Shibata,DK;Cote,RJ;Roy-Burman,P
通讯作者：
Roy-Burman,P
影响因子：
56.9
作者：
HOLLSTEIN, M;SIDRANSKY, D;HARRIS, CC
通讯作者：
HARRIS, CC
影响因子：
56.9
作者：
BAKER, SJ;MARKOWITZ, S;VOGELSTEIN, B
通讯作者：
VOGELSTEIN, B
影响因子：
3.5
作者：
D. Bostwick
通讯作者：
D. Bostwick
人类前列腺癌标本中的 p53 癌基因突变。
DOI：
10.1016/s0022-5347(17)35000-0
发表时间：
1994
期刊：
The Journal of urology
影响因子：
--
作者：
Voeller,HJ;Sugars,LY;Pretlow,T;Gelmann,EP
通讯作者：
Gelmann,EP