LC-Q-TOF-MS Characterization of Polyphenols from White Bayberry Fruit and Its Antidiabetic Effect in KK-Ay Mice
LC-Q-TOF-MS Characterization of Polyphenols from White Bayberry Fruit and Its Antidiabetic Effect in KK-Ay Mice
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白杨梅多酚的 LC-Q-TOF-MS 表征及其对 KK-Ay 小鼠的抗糖尿病作用
DOI：
10.1021/acsomega.0c02759
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发表时间：
2020-07-21
期刊：
影响因子：
4.1
通讯作者：
Li, Xian
中科院分区：
文献类型：
--
作者：
Liu, Yilong;Zhang, Xianan;Li, Xian
本研究旨在研究白肉杨梅品种“水井”的多酚成分及其体内抗糖尿病作用。采用液相色谱-四极杆飞行时间质谱（LC-TOF- MS）技术，从水井果提取物（SJE）中鉴定出38种多酚类物质，其中以表没食子儿茶素没食子酸酯（EGCG）为主要成分的原花青素（PAs）和杨梅苷、槲皮素为主要成分的黄酮醇类化合物。经过5周的实验，SJE （200 mg/kg体重）显著降低糖尿病KK-A(y)小鼠的空腹血糖，提高葡萄糖耐量和胰岛素敏感性。它能显著减轻小鼠体重增加，降低糖脂代谢相关标志物，包括胰岛素、瘦素、胰高血糖素、甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-c）和丙氨酸转氨酶（ALT）水平。SJE也显著降低了肝脏重量和肝脏脂质积累。SJE处理小鼠肝脏中胰岛素1 （INS1）和糖原合成酶激酶3 β (GSK3b)的基因表达被显著抑制，AMPK α的肝脏磷酸化水平显著升高，提示SJE可能通过AMPK依赖性途径发挥降糖作用。综上所述，富含PAs和黄酮醇的杨梅在糖尿病的调节中可能具有很大的潜力。
The present study is to investigate the polyphenolic composition and in vivo antidiabetic effect of white-fleshed Chinese bayberry cultivar "Shui Jing". By liquid chromatography quadrupole time-of-flight mass spectrometry (LC-TOF- MS), 38 polyphenols were identified in the Shui Jing fruit extract (SJE), where proanthocyanidins (PAs), including epigallocatechin gallate (EGCG), as well as flavonols, including myricitrin and quercetrin, were the predominant ingredients. After a 5-week experiment, the SJE (200 mg/kg bodyweight) significantly reduced fasting blood glucose, elevated glucose tolerance, and insulin sensitivity in diabetic KK-A(y) mice. It markedly attenuated bodyweight gain and decreased glycolipid metabolism-related markers including insulin, leptin, glucagon, triglyceride (TG), total cholesterol (TC), lowdensity lipoprotein cholesterol (LDL-c) and alanine aminotransferase (ALT) levels in mice. Liver weight and hepatic lipid accumulation were also significantly reduced by the SJE. Gene expressions of insulin 1 (INS1) and glycogen synthase kinase 3 beta (GSK3b) were markedly inhibited while the hepatic phosphorylation of AMPK alpha was significantly increased in the liver of SJE-treated mice, indicating that the SJE may exert an antidiabetic effect through an AMPK-dependent pathway. In conclusion, white bayberry rich in PAs and flavonols may have great potential in the regulation of diabetes mellitus.