Target genes regulated by transcription factor E2F1 in small cell lung cancer
Target genes regulated by transcription factor E2F1 in small cell lung cancer
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小细胞肺癌中受转录因子 E2F1 调控的靶基因
DOI：
10.13294/j.aps.2016.0043
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发表时间：
2016-06-25
期刊：
影响因子：
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通讯作者：
Kong Li-Jun
中科院分区：
文献类型：
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作者：
Li Zun-Ling;Jiao Fei;Kong Li-Jun
此前，我们已经报道了转录因子E2F1在大约95%的小细胞肺癌组织中表达上调，并与肿瘤的侵袭转移密切相关，但很少有研究研究E2F1调控的特定靶基因在小细胞肺癌中的作用。本研究旨在阐明小细胞肺癌细胞株H1688中E2F1调控的靶基因。染色质免疫沉淀测序结果显示，共鉴定出5326个潜在的靶基因，其中4700个为结构基因，626个为长非编码RNA(LncRNAs)。基因本体论(GO)和富集图分析结果表明，这些靶基因与三个主要功能相关：(1)细胞周期调节；(2)染色质和组蛋白修饰；(3)蛋白质转运。用MEME4.7.0软件鉴定E2F1结合DNA基序，发现6个基序编码基因和lncRNAs。这些结果阐明了E2F1的靶基因，为进一步探讨E2F1在小细胞肺癌的发生、发展、侵袭转移、复发和耐药中的作用提供了实验基础。
Previously, we have reported that transcription factor E2F1 expression is up-regulated in approximately 95% of small cell lung cancer tissue samples and closely associated with invasion and metastasis, but few studies have investigated specific target genes regulated by E2F1 in this disease. The aim of this study was to clarify the target genes controlled by E2F1 in the small cell lung cancer cell line H1688. The results of chromatin immunoprecipitation sequencing (ChIP-seq) showed that total 5 326 potential target genes were identified, in which 4 700 were structural genes and 626 long non-coding RNAs (lncRNAs). Gene Ontology (GO) and enrichment map analysis results indicated that these target genes were associated with three main functions: (1) cell cycle regulation, (2) chromatin and histone modification, and (3) protein transport. MEME4.7.0 software was used to identify the E2F1 binding DNA motif, and six motifs were discovered for coding genes and lncRNAs. These results clarify the.target genes of E2F1, and provide the experimental basis for further exploring the roles of E2F1 in tumorigenesis, development, invasion and metastasis, recurrence, and drug resistance in small cell lung cancer.