Mechanical strain regulates endothelial cell patterning in vitro
Mechanical strain regulates endothelial cell patterning in vitro
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DOI：
10.1089/ten.2006.0058
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发表时间：
2007-01-01
影响因子：
--
通讯作者：
Mooney, David J.
中科院分区：
文献类型：
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作者：
Matsumoto, Takuya;Yung, Yu Ching;Mooney, David J.
脊椎动物循环系统的血管通常表现出组织特异性模式。然而，指导这些模式发展的线索仍然不清楚。我们研究了在生长因子的影响下，在二维（2D）和三维（3D）培养系统中，周期性单轴应变对血管内皮细胞动力学和芽形成的影响。在单层培养中，细胞优先沿垂直于应变主轴的方向排列和运动，而在二维培养中，机械应变也调节细胞增殖的空间位置。通过适当的生长因子组合（血管内皮生长因子和肝细胞生长因子）可以诱导3D细胞培养中的细胞形成芽，并且应变方向调节该过程的方向性。此外，循环单轴应变抑制内皮细胞形成的结构的分支，并增加其厚度。总之，这些数据支持外部机械刺激在调节内皮细胞迁移、增殖和分化为原始血管中的重要性。
Blood vessels of the vertebrate circulatory system typically exhibit tissue-specific patterning. However, the cues that guide the development of these patterns remain unclear. We investigated the effect of cyclic uniaxial strain on vascular endothelial cell dynamics and sprout formation in vitro in two-dimensional (2D) and three-dimensional (3D) culture systems under the influence of growth factors. Cells preferentially aligned and moved in the direction perpendicular to the major strain axis in monolayer culture, and mechanical strain also regulated the spatial location of cell proliferation in 2D cell culture. Cells in 3D cell culture could be induced to form sprouts by exposure to appropriate growth factor combinations (vascular endothelial growth factor and hepatocyte growth factor), and the strain direction regulated the directionality of this process. Moreover, cyclic uniaxial strain inhibited branching of the structures formed by endothelial cells and increased their thickness. Taken together, these data support the importance of external mechanical stimulation in the regulation of endothelial cell migration, proliferation, and differentiation into primitive vessels.