A bioseparation apparatus with high-pressure fluid injection and fluid sampling.
A bioseparation apparatus with high-pressure fluid injection and fluid sampling.
复制标题
一种具有高压流体注射和流体采样的生物分离装置。
DOI：
10.1006/abio.1998.2946
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发表时间：
1999
影响因子：
2.9
通讯作者：
LaugharnJr,JA
中科院分区：
文献类型：
--
作者：
Dreier,GH;Tao,F;Hess,RA;Cheung,CY;Sciaba,LE;Green,DJ;LaugharnJr,JA
A novel apparatus in which fluids may be injected and sampled at high pressure is described. Bioseparation applications of the apparatus were demonstrated in three model systems: (1) λDNA was eluted under pressure from an anion exchange column into a low-salt (0.25 M) buffer, thereby eliminating conventional time-consuming desalting procedures required for downstream analysis of the DNA; (2) RNA was separated under pressure from a RNA/DNA mixture, thereby enabling rapid differential preparation of nucleic acids; and (3) an antibody was purified from a protein mixture by affinity capture at one pressure and dissociation from the antigen binding partner at a second pressure, thereby enabling the immunoreactivities of both antibody and antigen to be preserved during the separation process.
