Ultrastructural immunolocalization of laminin 332 (laminin 5) at dento-gingival interface in Macaca fuscata monkey
Ultrastructural immunolocalization of laminin 332 (laminin 5) at dento-gingival interface in Macaca fuscata monkey
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DOI：
10.1007/s00795-014-0085-9
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发表时间：
2015-06
影响因子：
1.8
通讯作者：
T. Sawada;Takaki Yamazaki;K. Shibayama;Y. Yamaguchi;M. Ohshima
中科院分区：
文献类型：
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作者：
T. Sawada;Takaki Yamazaki;K. Shibayama;Y. Yamaguchi;M. Ohshima
虽然层粘连蛋白 332（层粘连蛋白 5）是一种参与细胞粘附和迁移的细胞外基质分子，已定位于牙釉质和交界上皮之间的界面，但其超微结构定位仍有待完全阐明。本研究的目的是使用嵌入前和嵌入后免疫电子显微镜研究日本猴（Macaca fuscata）牙-牙龈界面处层粘连蛋白 332 的超微结构分布。预包埋免疫电子显微镜显示了宽阔的内部基底层和补充的致密层，并且均显示出层粘连蛋白 332 的免疫标记。在靠近牙釉质的交界上皮细胞的粗糙表面内质网中观察到层粘连蛋白 332 的免疫反应产物。包埋后免疫电子显微镜显示，朝向冠状部分的免疫金颗粒数量增加，导致颗粒在基底层（尤其是致密层）上大量积累。同时，牙齿-牙龈界面处的牙齿角质层偶尔但特别地用抗层粘连蛋白 332 抗体进行免疫金标记。这些数据表明，交界上皮活跃地产生层粘连蛋白332，并且在细胞冠向牙龈沟迁移期间，产物在牙-牙龈界面处积累。
Although laminin 332 (laminin 5), an extracellular matrix molecule involved in cell adhesion and migration, has been localized at the interface between the tooth enamel and junctional epithelium, its ultrastructural localization remains to be fully clarified. The purpose of the present study was to investigate the ultrastructural distribution of laminin 332 at the dento-gingival interface in Japanese monkey (Macaca fuscata) using pre- and post-embedding immunoelectron microscopy. Pre-embedding immunoelectron microscopy revealed a broad band of internal basal lamina together with supplementary lamina densa, and both showed immunolabeling for laminin 332. Immunoreaction products for laminin 332 were observed in the rough-surfaced endoplasmic reticulum of the junctional epithelial cells close to the tooth enamel. Post-embedding immunoelectron microscopy revealed an increase in the number of immunogold particles toward the coronal portion, resulting in a large accumulation of particles on the basal lamina, preferentially on the lamina densa. Concomitantly the dental cuticle at the dento-gingival interface was sporadically, but specifically, immunogold-labeled with anti-laminin 332 antibody. These data suggest that junctional epithelium actively produces laminin 332, and that the products accumulate at the dento-gingival interface during cell migration coronally towards the gingival sulcus.