Immunotyping of Chlamydia trachomatis with monoclonal antibodies.
Immunotyping of Chlamydia trachomatis with monoclonal antibodies.
复制标题
用单克隆抗体对沙眼衣原体进行免疫分型。
DOI：
10.1093/infdis/152.4.791
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Grayston,JT
中科院分区：
文献类型：
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作者：
Wang,SP;Kuo,CC;Barnes,RC;Stephens,RS;Grayston,JT
用单克隆抗体对沙眼衣原体进行微量免疫荧光检测，可获得快速、准确的沙眼衣原体免疫分型。通过使用促进免疫型特异性抗体发展的免疫方案，用标准技术产生单克隆抗体。选择了15种单型或多型（亚种）单克隆抗体用于两步分型系统，该系统需要C.沙眼是针对6至8个单克隆抗体进行测试的分类。通过检测313株菌株（使用免疫小鼠抗血清的先前方法分型，代表15个已确定血清型中的每一个），研究了单克隆抗体免疫分型。两步单克隆抗体方法导致与当前方法相似的分类。只有一个菌株用两种方法分型不同。用单克隆抗体方法，可用的性病性淋巴肉芽肿（LOV）血清型L1和L3不能分别与沙眼血清型E和G区分，除非菌株已被鉴定为LOV。单克隆抗体分型更简单，更精确，它可以很容易地区分密切相关的血清型。在先前分类为血清型D、I和L2的菌株中发现了三种新类型。这些暂时被认为是亚型，并标记为D′，I′和L，′。
Rapid and precise immunotyping ofChlamydia trachomatiswas achieved by testing whole organisms (elementary bodies) in the microimmunofluorescence test with monoclonal antibodies. Monoclonal antibodies were produced with standard techniques by using an immunization schedule that encouraged the development of immunotype-specific antibodies. Fifteen mono typic or multitypic (subspecies) monoclonal antibodies were chosen for use in a two-step typing system that required strains ofC. trachomatisto be tested against six to eight monoclonal antibodies for classification. Immunotyping with monoclonal antibodies was studied by testing 313 strains, typed with the previous method that utilized immunized mouse antisera, that represented each of the 15 established serovars. The two-step monoclonal antibody method resulted in a classification similar to the current one. Only one strain typed differently with the two methods. With the monoclonal antibody method, available lymphogranuloma venereum (LOV) serovars L1 and L3 could not be differentiated from trachoma serovars E and G, respectively, unless the strains had been identified as LOV. Monoclonal antibody typing was simpler to perform and more precise; it allowed easy differentiation between closely related serovars. Three new types were discovered among the strains previously classified as serovars D, I, and L2. These are tentatively being considered subtypes and are labeled D′, I′, and L,′.