Volatile organic compounds from endophytic fungi as innovative postharvest control of Fusarium oxysporum in cherry tomato fruits
Volatile organic compounds from endophytic fungi as innovative postharvest control of Fusarium oxysporum in cherry tomato fruits
复制标题
DOI：
10.1007/s00253-017-8542-8
复制
发表时间：
2017-11-01
影响因子：
5
通讯作者：
Lydia Macias-Rubalcava, Martha
中科院分区：
文献类型：
--
作者：
Mariana Medina-Romero, Yoli;Roque-Flores, Gonzalo;Lydia Macias-Rubalcava, Martha
为了评估它们作为生物农药的潜力，在体内和体外研究了来自内生真菌的六种挥发性有机化合物对植物病原体尖孢镰刀菌生长的影响;化合物作为混合物和单独使用。采用琼脂稀释法和气相色谱法研究了樱桃番茄对病原菌的体内抑菌效果。此外，菌丝暴露于这些化合物的形态进行了分析。此外，通过研究呼吸作用和细胞膜通透性，确定了这些化合物的可能作用机制。结果表明，这些化合物具有显着的浓度依赖性的抗真菌作用的单独和协同作用的方式。此外，还观察到细胞膜透性的变化、菌丝形态的损伤和对呼吸的抑制作用。这6种化合物的混合物可用于采后防治。番茄中的尖孢菌。
To assess their potential as biopesticides, the effect on the growth of phytopathogen Fusarium oxysporum of six volatile organic compounds from endophytic fungi was studied in vivo and in vitro; compounds were used both as a mixture and individually. In vivo studies were performed inoculating the pathogen into cherry tomatoes, while the in vitro antifungal effect was studied using agar dilution and gas phase methods. Also, the morphology of the hyphae exposed to these compounds was analyzed. Moreover, the possible mechanism of action of these compounds was determined by studying the respiration and cell membrane permeability. Results show that the compounds have a significant concentration-dependent antifungal effect individually and act in a synergic manner. Additionally, changes in cell membrane permeability, damage to the hyphal morphology, and an inhibitory effect on the respiration were observed. The mixture of the six compounds may be used for postharvest control of F. oxysporum in tomatoes.