A MODIFIED BIOASSAY FOR ENERGY-UTILIZATION IN NEWLY-HATCHED CHICKS .2. DETERMINATION OF FEED INPUT AND PROCEDURES TO ESTIMATE ENDOGENOUS ENERGY-LOSS
A MODIFIED BIOASSAY FOR ENERGY-UTILIZATION IN NEWLY-HATCHED CHICKS .2. DETERMINATION OF FEED INPUT AND PROCEDURES TO ESTIMATE ENDOGENOUS ENERGY-LOSS
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DOI：
10.3382/ps.0740343
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发表时间：
1995-02-01
期刊：
影响因子：
4.4
通讯作者：
HORIGUCHI, M
中科院分区：
文献类型：
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作者：
MURAKAMI, H;AKIBA, Y;HORIGUCHI, M
本研究在TME生物测定系统的基础上，设计了一种新的生物测定系统，用于测定雏鸡日粮能量的生物利用率。 分别对0、1、3、7和14日龄的肉仔鸡进行强制喂食或在停喂24 h后自由采食饲料，以确定饲料投入的最佳水平。 试验二对7日龄雏鸡在禁饲24 h后，按3个水平分别饲喂不同的日粮和饲料，试验一和试验二的能量输入（X）和排泄能（Y）之间均呈线性回归（P <0.01），与日粮和饲料无关。 内源性能量损失（EEL），估计的回归方法和近似在一个小范围内低于EEL估计使用饲料剥夺小鸡。用回归法估计的EEL计算的日粮和饲料的TME和TME（n）值不受饲料投入水平的调节，而由饲料剥夺雏鸡估计的TME和TME（n）值则随饲料投入水平的降低而增加。 因此，提出了一种基于TME测定的新出壳雏鸡能量利用率测定方法，该方法具有一定的生理和化学基础。
Two experiments were conducted to develop a modified bioassay system to determine bioavailability of dietary energy in newly hatched chicks based on the TME assay system. Broiler chicks aged 0, 1, 3, 7, and 14 d old were force-fed or consumed feed ad libitum following 24 h of feed withdrawal to determine the optimum level for feed input. In the second experiment, 7-d-old chicks were force-fed various diets and feedstuffs at three levels following 24 h of feed deprivation.Linear regressions (P < .01) between energy input (X) and excreted energy (Y) were demonstrated irrespective of diets and feedstuffs at any age in Experiments 1 and 2. Endogenous energy loss (EEL), estimated by the regression method and approximated over a small range was lower than EEL estimated using feed-deprived chicks. The TME and TME(n) values of diets and feedstuffs calculated using EEL estimated by the regression method were not modulated by the feed input level, whereas those estimated by feed-deprived chicks tended to increase with decreases in the level of feed input. Thus, a modified method for determination of energy utilization in newly hatched chicks based on TME assay is proposed, which seems to have proper physiological and chemical bases.