Localization of 5-hydroxytryptamine1A and 5-hydroxytryptamine7 receptors in rabbit ocular and brain tissues
Localization of 5-hydroxytryptamine1A and 5-hydroxytryptamine7 receptors in rabbit ocular and brain tissues
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DOI：
10.1016/s0306-4522(98)00181-x
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发表时间：
1998-12-01
期刊：
影响因子：
3.3
通讯作者：
Osborne, NN
中科院分区：
文献类型：
--
作者：
Chidlow, G;Le Corre, S;Osborne, NN
5-羟色胺被认为在兔眼的各种组织中发挥着生理作用，但人们对不同的5-羟色胺受体的相对分布知之甚少。为了了解5-羟色胺在眼睛中的功能，对存在于各种眼组织中的受体亚型进行论证是必不可少的。用原位杂交组织化学、体外受体放射自显影和聚合酶链式反应相结合的方法，研究了5-羟色胺(1A)和5-羟色胺(7)受体在兔眼中的分布。由于这些受体在兔体内还没有测序，我们最初通过对脑组织的分析确定了寡核苷酸探针的适用性。5-羟色胺(1A)和5-羟色胺(7)受体信使RNA在兔脑中的分布与其他物种的分布有很好的相关性，证实了该探针在兔组织中检测信使RNA的特异性。在眼睛中，5-羟色胺(1A)受体的表达仅限于睫状突的上皮细胞层，尽管在视网膜中可能出现非常低的水平。相比之下，5-羟色胺(7)受体信使RNA的表达更为广泛，在睫状突、视网膜和虹膜中有明显的阳性信号。结果证实了5-羟色胺(1A)受体在睫状体中的存在及其在睫状体上皮细胞中的定位，支持了它们参与房水体液分泌的假说。出乎意料的是，几乎没有证据支持5-羟色胺(1A)受体存在于视网膜和虹膜括约肌的观点。然而，随后在视网膜和虹膜中发现的5-羟色胺(7)受体信使RNA可能解释了这些组织中明显缺乏5-羟色胺(1A)受体的原因。睫状突中5-羟色胺(1A)和5-羟色胺(7)受体的存在可能解释了兔对5-羟色胺的复杂眼压反应。(C)1998年IBRO。爱思唯尔科学有限公司出版。
Serotonin is thought to play a physiological role in various tissues of the rabbit eye, yet little is known about the relative distribution of the different serotonin receptors. Demonstration of the receptor subtypes present in the various ocular tissues is essential in order to understand the function of serotonin in the eye. Using a combination of in situ hybridization histochemistry, in vitro receptor autoradiography and polymerase chain reaction studies, rye have explored the distribution of the 5-hydroxytryptamine(1A) and 5-hydroxytryptamine(7) receptors in the rabbit eye. As these receptors have not been sequenced in the rabbit, we initially established the suitability of the oligonucleotide probes by analysis of brain tissue. The distributions of 5-hydrosytryptamine(1A) and 5-hydroxytryptamine(7) receptor messenger RNAs in rabbit brain correlated well with those in other species, confirming the specificity of the probes for detection of the messenger RNAs in rabbit tissues. In the eye, the expression of 5-hydroxytryptamine(1A) receptors arrears to be restricted to the epithelial cell layer of the ciliary processes, although very low levels may appear in the retina. In contrast, the expression of 5-hydroxytryptamine(7) receptor messenger RNA is more widespread with positive signals evident in the ciliary processes, retina and iris.The results confirm the existence of 5-hydroxytryptamine(1A) receptors in the ciliary body and their localization in the ciliary epithelium supports the hypothesis that they are involved in the secretion of aqueous humour. Unexpectedly, there was little evidence to support the idea that 5-hydroxytryptamine(1A) receptors are present in the retina and iris sphincter. However, the subsequent finding of 5-hydroxytryptamine(7) receptor messenger RNA in the retina and iris may explain the apparent absence of 5-hydroxytryptamine(1A) receptors in these tissues. The presence of both 5-hydroxytryptamine(1A) and 5-hydroxytryptamine(7) receptors in the ciliary processes may account for the complex intraocular pressure response of the rabbit to seratonin. (C) 1998 IBRO. Published by Elsevier Science Ltd.