THE TEMPORAL AND SPATIAL-DISTRIBUTION PATTERN OF MATERNAL EXUPERANTIA PROTEIN - EVIDENCE FOR A ROLE IN ESTABLISHMENT BUT NOT MAINTENANCE OF BICOID MESSENGER-RNA LOCALIZATION
THE TEMPORAL AND SPATIAL-DISTRIBUTION PATTERN OF MATERNAL EXUPERANTIA PROTEIN - EVIDENCE FOR A ROLE IN ESTABLISHMENT BUT NOT MAINTENANCE OF BICOID MESSENGER-RNA LOCALIZATION
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DOI：
10.1002/j.1460-2075.1991.tb05004.x
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发表时间：
1991-12-01
期刊：
影响因子：
11.4
通讯作者：
HAZELRIGG, T
中科院分区：
文献类型：
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作者：
MARCEY, D;WATKINS, WS;HAZELRIGG, T
黑腹果蝇的exuperantia（exu）基因通过确保bicoid（bcd）基因的mRNA正确定位于卵母细胞的前部区域，在卵母细胞和早期胚胎的极性建立中发挥着重要作用。我们已经分离并测序了exu基因，测序了其女性特异性转录本和一个主要影响exu女性生殖系功能的exu突变等位基因，并确定了卵子发生过程中exu蛋白表达的时空模式。exu蛋白是碱性的，至少有一个碱性残基被鉴定为雌性生殖系中exu功能所必需的，并且在卵子发生期间短暂存在。我们的研究结果表明，exu是不需要的bcd mRNA定位在卵子发生的晚期和早期胚胎发生的维护，而是为建立bcd mRNA定位在发育中的卵母细胞。我们认为exu蛋白可能是用来修饰bcd mRNA结合的成分，或者修饰bcd信息本身，或者在bcd mRNA在发育卵母细胞定位位点的对接中发挥作用。
The exuperantia (exu) gene of Drosophila melanogaster plays a fundamental role in the establishment of polarity of the oocyte and early embryo by ensuring the proper localization of the mRNA of the bicoid (bcd) gene to anterior regions of the oocyte. We have isolated and sequenced the exu gene, sequenced its female-specific transcript and a mutant allele of exu that affects primarily exu's female germline function, and determined the temporal and spatial pattern of exu protein expression during oogenesis. The exu protein is basic, with at least one basic residue being identified as necessary for exu function in the female germline, and is present transiently during oogenesis. Our results suggest that exu is not required for the maintenance of bcd mRNA localization during late stages of oogenesis and early embryogenesis, but rather for the establishment of bcd mRNA localization in the developing oocyte. We propose that the exu protein may serve to modify a component that binds bcd mRNA or to modify the bcd message itself, or may perform a role in docking the bcd mRNA at its site of localization in the developing oocyte.