SERUM NEUTRALIZING ANTIBODY-RESPONSE TO HEPATITIS-A VIRUS
SERUM NEUTRALIZING ANTIBODY-RESPONSE TO HEPATITIS-A VIRUS
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DOI：
10.1093/infdis/148.6.1033
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发表时间：
1983-01-01
影响因子：
6.4
通讯作者：
BINN, LN
中科院分区：
文献类型：
--
作者：
LEMON, SM;BINN, LN
用放射自显影法测定了实验感染的灵长类动物和自然感染的人血清中甲型肝炎病毒（HAV）的中和抗体。用甲型肝炎病毒的PA-33或HM-175株感染灵长类动物，可引起能够中和任一株的抗体。对2只猴的连续检测表明，中和抗体与免疫测定法检测到的抗体密切相关，在肝酶升高之前产生，并与病毒抗原粪便脱落的大幅减少相关。在对甲型肝炎暴发的人类受试者进行的试验中，中和抗体在症状发作前3-5天存在，并在19 S [沉降系数]和7 S IG组分中发现。对HAV的免疫力可能主要是由于中和抗体;定量这种抗体的能力将有助于评价新的HAV疫苗。
Serum neutralizing antibody to hepatitis A virus (HAV) was measured in experimentally infected primates and naturally infected humans by means of an assay based on the autoradiographic detection of viral replication foci in vitro. Infection of primates with PA-33 or HM-175 strains of HAV elicited antibody capable of neutralizing either strain. Sequential testing of 2 monkeys showed that neturalizing antibody correlated closely with antibody detected by immunoassay, developed before liver enzyme elevations, and was associated with a substantial reduction in fecal shedding of viral antigen. In tests performed on human subjects involved in an outbreak of hepatitis A, neutralizing antibody was present 3-5 days before the onset of symptoms and was found in 19S [sedimentation coefficient] and 7S Ig fractions. Immunity to HAV is probably due primarily to neutralizing antibody; the ability to quantitate this antibody will be helpful in the evaluation of new HAV vaccines.