GENETIC-ANALYSIS OF STUNTED GROWTH BY NUCLEAR-CYTOPLASMIC INTERACTION IN INTERSPECIFIC HYBRIDS OF CAPSICUM BY USING RAPD MARKERS
GENETIC-ANALYSIS OF STUNTED GROWTH BY NUCLEAR-CYTOPLASMIC INTERACTION IN INTERSPECIFIC HYBRIDS OF CAPSICUM BY USING RAPD MARKERS
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DOI：
10.1007/bf00215086
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发表时间：
1993-12-01
影响因子：
5.4
通讯作者：
IKEHASHI, H
中科院分区：
文献类型：
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作者：
INAI, S;ISHIKAWA, K;IKEHASHI, H
以8个辣椒品种为母本，与2个辣椒品种进行种间杂交，结果表明：16个组合中有10个组合出现矮化现象，其余6个组合的杂种生长正常。相反，当C. chinense为母本，所有杂种均表现出严重的生长迟缓，似乎受到病毒的影响。这些结果表明，生长发育迟缓表现在玉米杂交中。chinense x C. annuum是由C. annuum和C. chinense。为了检测引起生长迟缓的核基因的数目，我们将C. annuum × C. chinense和C. chinense。回交后代中约有四分之一的后代表现出明显的早熟性。chinense x（C. annuum × C. chinense）的F1代表现出相同的生长迟缓现象。chinense x C. annuum的两个互补基因，推测C.一年生植物导致生长迟缓。但C.胚珠败育率较高，种子萌发率较低。chinense x C. annuum比自交的C. chinense的遗传多样性表明，发育迟缓型的遗传比例可能高于中国对虾。chinense x（C. annuum × C. chinense）后代。然后，我们尝试了生长迟缓与随机扩增多态性DNA（RAPD）之间的连锁分析。chinense x（C. annuum × C. chinense）后代。一个RAPD标记，与94%的矮化植物，但不与94%的正常的一个。这证实了C.与RAPD标记连锁的一年生菜豆（C.annuum）在与菜豆细胞质互作中表现出生长受阻的现象，其重组率为6%。chinense。
When eight cultivars of Capsicum annuum were used as female parents in interspecific crosses with two accessions of C. chinense, dwarfism occurred in hybrids originating from 10 out of 16 combinations, while hybrids of the remaining 6 combinations grew normally. In contrast, when C. chinense was used as female parent, all of the hybrids showed severely stunted growth as if affected by a virus. These results suggested that the stunted growth expressed in the cross of C. chinense x C. annuum is caused by an interaction between nuclear gene(s) from C. annuum and the cytoplasm of C. chinense. To examine the number of nuclear gene(s) which cause(s) the stunted growth, we backcrossed F1 hybrids of C. annuum x C. chinense to C. chinense. About one-quarter of the progeny in the backcrossed hybrids of C. chinense x (C. annuum x C. chinense) showed the same stunted growth shown by the F1 hybrids of C. chinense x C. annuum, suggesting that two complementary genes of C. annuum cause the stunted growth. However, the higher abortion rates of ovules and lower germination percentage of seeds in C. chinense x C. annuum than in the selfed C. chinense implied that the genetic ratio of the stunted type would have been higher than that observed in the C. chinense x (C. annuum x C. chinense) progeny. We then attempted a linkage analysis between the stunted growth and randomly amplified polymorphic DNA (RAPD) of C. chinense x (C. annuum x C. chinense) progeny. A RAPD marker that associated with 94% of the stunted plants but not with 94% of the normal one was identified. This confirmed that a single nuclear gene of C. annuum which is linked to the RAPD marker with a recombination value of 6% causes the stunted growth in an interaction with the cytoplasm of C. chinense.