Neonates colonized with pathogenic bacteria in the airways have a low-grade systemic inflammation
Neonates colonized with pathogenic bacteria in the airways have a low-grade systemic inflammation
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DOI：
10.1111/all.13461
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发表时间：
2018-11-01
期刊：
影响因子：
12.4
通讯作者：
Bisgaard, H.
中科院分区：
文献类型：
--
作者：
Fink, N. Rahman;Chawes, B. L.;Bisgaard, H.
背景与目的儿童哮喘的发生与新生儿下咽部病原菌定植有关。此外，已建立的哮喘与全身性低度炎症有关。我们在此报告了新生儿下咽部致病菌定植与全身性低度炎症发展之间的关系。方法下咽部的细菌定植有莫拉氏菌、流感嗜血杆菌、和/或肺炎链球菌在哥本哈根儿童哮喘前瞻性研究（2000年）中的无症状儿童中进行了评估（COPSAC（2000））队列1个月龄时通过培养技术（N = 238）和定量聚合酶链反应（qPCR）技术（N = 249）和COPSAC（2010）队列，在1月龄时培养（N = 622）并在3月龄时再次培养（N = 613）。通过测量高敏C反应蛋白（hs-CRP）、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的血浆水平，在6个月大时确定两个组群中的全身性低度炎症。结果在两个队列中，细菌定植与hs-CRP水平升高相关：COPSAC（2000），1个月培养（定殖/非定殖的几何平均比率[95%CI]），1.39 [0.97-2.01]，P = 0.08; 1个月qPCR，1.55 [1.14-2.10]，P <0.01; COPSAC（2010），1个月，1.52 [1.23-1.87]，P < .01; 3个月，1.57 [1.30-1.90]，P < .01。结合hs-CRP、TNF-α和IL-6的多参数主成分分析证实了M定植儿童的全身炎症特征。catharralis，H.流感。和/或S.与非定植儿童相比，下咽部中的肺炎（P值<0.05）。结论生命早期上呼吸道微生物组的组成可能导致全身性低度炎症。
Background and objectives The development of childhood asthma is associated with neonatal colonization with pathogenic bacteria in hypopharynx. Furthermore, established asthma is associated with systemic low-grade inflammation. We here report on the association between neonatal colonization with pathogenic bacteria in hypopharynx and the development of systemic low-grade inflammation. Methods Bacterial colonization of the hypopharynx with Moraxella catharralis, Haemophilus influenzae, and/or Streptococcus pneumoniae was assessed in asymptomatic children from the Copenhagen Prospective Studies on Asthma in Childhood(2000) (COPSAC(2000)) cohort at age 1 month by culturing technique (N = 238) and by quantitative polymerase chain reaction (qPCR) technique (N = 249) and in the COPSAC(2010) cohort by culturing at age 1 month (N = 622) and again at age 3 months (N = 613). Systemic low-grade inflammation was determined in both cohorts at age 6 months by measuring plasma levels of high-sensitivity C-reactive protein (hs-CRP), tumor necrosis factor-alpha (TNF-alpha), and interleukin-6 (lL-6). Results In both cohorts, bacterial colonization was associated with increased levels of hs-CRP: COPSAC(2000), 1 month culturing (geometric mean ratio of colonized/noncolonized [95% CI]), 1.39 [0.97-2.01], P = .08; 1 month qPCR, 1.55 [1.14-2.10], P < .01; COPSAC(2010), 1 month, 1.52 [1.23-1.87], P < .01; and 3 month, 1.57 [1.30-1.90], P < .01. A multiparametric principal component analysis incorporating hs-CRP, TNF-alpha, and IL-6 confirmed a systemic inflammatory profile in children colonized with M. catharralis, H. influenzae. and/or S. pneumoniae in the hypopharynx compared to noncolonized children (P-values < .05). Conclusion The composition of the upper airway microbiome in early life may cause systemic low-grade inflammation.