Comparison of salicylate- and quinine-induced tinnitus in rats: development, time course, and evaluation of audiologic correlates.
Comparison of salicylate- and quinine-induced tinnitus in rats: development, time course, and evaluation of audiologic correlates.
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DOI：
10.1097/mao.0b013e3181de4662
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发表时间：
2010-07
期刊：
影响因子：
--
通讯作者：
Salvi R
中科院分区：
文献类型：
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作者：
Ralli M;Lobarinas E;Fetoni AR;Stolzberg D;Paludetti G;Salvi R
水杨酸盐和奎宁已被证明可靠地诱导短期耳鸣时，在高剂量管理。本研究采用声惊吓间隙差距前脉冲抑制法（GPIAS）比较水杨酸和奎宁诱发的大鼠耳鸣。将24只大鼠分为两组，第一组（n=12）注射水杨酸（300 mg/kg/d），第二组（n=12）口服奎宁（200 mg/kg/d）。动物每天给药，连续4天。在首次给药前及给药后2、24、48、72、96 h检测耳鸣和听力损失。用GPIAS评估耳鸣，用DPOAE和ABR检测听功能。水杨酸盐治疗引起短暂性耳鸣，从治疗后2小时开始音调接近16 kHz，持续4天治疗期，24小时后消失。奎宁组动物在较高音调（20 kHz）下显示GPIAS变化;然而，动物间变化更大，平均数据无统计学显著性。听力功能因治疗而异。在水杨酸组中，高水平DPOAE受到轻微影响;大多数变化发生在治疗后2小时。低水平DPOAE在所有频率下均受到影响，并具有进行性剂量依赖性效应。在奎宁组中，只有高水平的DPOAE受到影响，主要是在16 kHz。本研究强调了水杨酸盐和奎宁引起的耳鸣和听力减退的频率和时程的相似性和差异。暂时性耳鸣可靠地诱导水杨酸盐，而听力损失仍然是亚临床的DPOAE只有微小的变化。
Salicylate and quinine have been shown to reliably induce short-term tinnitus when administered at high doses. The present study compared salicylate and quinine induced tinnitus in rats using the gap prepulse inhibition of acoustic startle (GPIAS). Twenty-four rats were divided into 2 groups; the first group (n=12) was injected with salicylate (300 mg/kg/d), the second (n=12) was treated with quinine orally at a dose of 200 mg/kg/d. Animals were treated daily for 4 consecutive days. All rats were tested for tinnitus and hearing loss before and 2, 24, 48, 72, 96 hours after the first drug administration. Tinnitus was assessed using GPIAS; hearing function was measured with DPOAEs and ABR. Salicylate treatment induced transient tinnitus with a pitch near 16 kHz starting 2 h post-treatment, persisting over the 4-day treatment period and disappearing 24 h later. Animals in the quinine group showed GPIAS changes at a higher pitch (20 kHz); however, changes were more variable among animals and the mean data were not statistically significant. Hearing function varied across treatments. In the salicylate group, high-level DPOAEs were slightly affected; most changes occurred 2 h post-treatment. Low-level DPOAEs were affected at all frequencies with a progressive dose-dependent effect. In the quinine group, only high-level DPOAEs were affected, mainly at 16 kHz. The present study highlights the similarities and differences in the frequency and the time course of tinnitus and hypoacusis induced by salicylate and quinine. Transient tinnitus was reliably induced pharmacologically with salicylate while hearing loss remained subclinical with only minor changes in DPOAEs.