Isolation and characterization of Wnt pathway-related genes from Porifera
Isolation and characterization of Wnt pathway-related genes from Porifera
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DOI：
10.1016/j.cellbi.2007.03.003
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发表时间：
2007-09-01
影响因子：
3.9
通讯作者：
Mueller, Werner E. G.
中科院分区：
文献类型：
--
作者：
Adell, Teresa;Thakur, Archana N.;Mueller, Werner E. G.
Wnt信号通过结合卷曲受体起作用，随后激活两种不同的信号转导级联，即经典和非经典Wnt途径，参与细胞生长、分化、迁移和命运。经典途径通过β-连环蛋白易位到细胞核和TCF/LEF转录因子的激活发挥作用;它在胚胎发生期间的发育模式和细胞命运决定中起重要作用。非经典的Wnt信号通路在无脊椎动物中负责平面细胞极性过程，并在脊椎动物原肠胚形成期间负责会聚-伸展运动。非经典Wnt途径的最终作用是通过Ras样小GTP酶亚家族的活化而使细胞骨架重排。在最近的一份报告中，我们首次描述了Wnt相关基因Sd-Frizzled的分离，从最基础的动物门，多孔动物门。在本研究中，我们报告了几个Wnt通路相关基因的分离和系统发育特征的海绵Suberites domuncula：Sd-TCF/LEF，Sd-GSK 3，一个最近发现的分子与一个假定的功能作为Wnt调节器（Sd-LZIC），小Rho GTP酶Sd-RhoA，Sd-Cdc 42，和它们的效应Sd-mrlc。还报道了从另一种海绵（Lubomirskia baicalensis）中分离到的分泌型卷曲相关蛋白sFRP。(C)2007年国际细胞生物学联合会。由爱思唯尔有限公司出版。保留所有权利。
The Wnt signal acts by binding to Frizzled receptors, with the subsequent activation of two different signal transduction cascades, the canonical and the non-canonical Wnt pathways, involved in cell growth, differentiation, migration and fate. The canonical pathway functions through the translocation of beta-catenin to the nucleus and the activation of TCF/LEF transcription factors; it plays an important role in developmental patterning and cell fate decisions during embryogenesis. The non-canonical Wnt pathway is responsible for the planar cell polarity process in invertebrates, and for the convergent-extension movements during vertebrate gastrulation. The final effect of the non-canonical Wnt pathway is the rearrangement of the cell cytoskeleton, through the activation of the subfamily of Ras-like small GTPases. In a recent report we described for the first time the isolation of a Wnt-related gene, Sd-Frizzled, from the most basal animal phylum, the Porifera. In the present study we report the isolation and phylogenetic characterization of several Wnt pathway-related genes from the sponge Suberites domuncula: Sd-TCF/LEF, Sd-GSK3, a recently discovered molecule with a putative function as a Wnt regulator (Sd-LZIC), the small Rho GTPases Sd-RhoA, Sd-Cdc42, and their effector Sd-mrlc. Also the isolation of a secreted frizzled related protein sFRP from another sponge species (Lubomirskia baicalensis) is reported. (C) 2007 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.