Shedding routes of Coxiella burnetii in dairy cows:: implications for detection and control
Shedding routes of Coxiella burnetii in dairy cows:: implications for detection and control
复制标题
DOI：
10.1051/vetres:2006038
复制
发表时间：
2006-11-01
影响因子：
4.4
通讯作者：
Seegers, Henri
中科院分区：
文献类型：
--
作者：
Guatteo, Raphael;Beaudeau, Francois;Seegers, Henri
伯内特立克克斯体脱落物的可靠检测是控制这种细菌在动物之间以及从动物到人类传播的关键点。伯内特柯克斯体主要由反刍动物通过分娩产物（胎盘、分娩液）排出，但也可能通过阴道粘液、乳汁、粪便、尿液和精液排出。然而，这些类型的样品在现场条件下识别脱落物的信息价值尚不清楚。我们的目标是描述使用实时 PCR 技术获得的反应，该技术应用于从商业奶牛群中已知自然感染伯内氏立克次体的 242 头奶牛采集的牛奶、阴道粘液和粪便样本，并评估它们的假定关联。在所有类型的测试样本中，甚至在粪便中，都发现了阳性结果。没有确定主要的脱落途径。在掉毛奶牛中，65.4%仅通过一种途径被检测为掉毛奶牛。相比之下，所有三个样本均呈阳性结果的奶牛很少（不到 7%）。仅基于一种类型的生物样本对奶牛进行测试可能会导致其在伯内氏立克次体脱落方面对其进行错误分类，从而低估细菌在牛群内传播的风险。
Reliable detection of Coxiella burnetii shedders is a critical point for the control of the spread of this bacterium among animals and from animals to humans. Coxiella burnetii is shed by ruminants mainly by birth products (placenta, birth fluids), but may also be shed by vaginal mucus, milk, and faeces, urine and semen. However, the informative value of these types of samples to identify shedders under field conditions is unknown. Our aim was then to describe the responses obtained using a real-time PCR technique applied to milk, vaginal mucus and faeces samples taken from 242 dairy cows in commercial dairy herds known to be naturally infected with Coxiella burnetii, and to assess their putative associations. Positive results were found in all types of tested samples even in faeces. No predominant shedding route was identified. Among the shedder cows, 65.4% were detected as shedders by only one route. By contrast, cows with positive results for all three samples were scarce (less than 7%). Testing a cow based on only one type of biological sample may lead to misclassify it with regards to its shedding of Coxiella burnetii and thereby underestimate the risk of bacterial spread within a herd.