Pel, the protein that permits lambda DNA penetration of Escherichia coli, is encoded by a gene in ptsM and is required for mannose utilization by the phosphotransferase system.
Pel, the protein that permits lambda DNA penetration of Escherichia coli, is encoded by a gene in ptsM and is required for mannose utilization by the phosphotransferase system.
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Pel 是一种允许 lambda DNA 穿透大肠杆菌的蛋白质，由 ptsM 中的基因编码，是磷酸转移酶系统利用甘露糖所必需的。
DOI：
10.1073/pnas.83.23.8934
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发表时间：
1986
影响因子：
11.1
通讯作者：
Roseman,S
中科院分区：
文献类型：
--
作者：
Williams,N;Fox,DK;Shea,C;Roseman,S
大肠杆菌中的甘露糖摄取和磷酸化由磷酸烯醇丙酮酸：糖磷酸转移酶系统 (PTS) 催化。 PTS 的甘露糖特异性复合物称为 IIMan，包含脂质和两种膜蛋白 II-AMan 和 II-BMan。这些蛋白质由 ptsM 编码，位于大肠杆菌染色体上约 40 分钟处。一种不同的遗传标记 pel 与 ptsM 作图，并且是 lambda DNA 穿透细胞质膜所必需的。早期研究表明 pel 功能和 II-BMan 均由同一基因编码，而不同的基因（也在 ptsM 中）编码 II-AMan。在本研究中，从 pBR322 中的大肠杆菌 HindIII 基因库中分离出 ptsM 克隆 pCS13，并恢复了 II-BMan 中缺陷的 ptsM 突变体的甘露糖终止和 pel+ 功能。 pCS13 的亚克隆显示两个不同的基因 manY 和 manZ 分别编码 pel+ 功能和 II-BMan 蛋白；每个基因可能有自己的启动子；虽然单独由 manY (Pel) 编码的蛋白质似乎足以满足 lambda 敏感性，但所有三种基因产物都是甘露糖发酵、甘露糖类似物 2-脱氧葡萄糖的转运以及后者通过细胞质膜磷酸化所必需的。因此，Pel 是 IIMan 复合体功能所必需的。复合物的效率可能取决于 Pel 与 IIMan 的比率。
Mannose uptake and phosphorylation in Escherichia coli is catalyzed by the phosphoenolpyruvate:glycose phosphotransferase system (PTS). The mannose-specific complex of the PTS, designated IIMan, comprises lipid and two membrane proteins, II-AMan and II-BMan. The proteins are encoded by ptsM, located at approximately equal to 40 minutes on the E. coli chromosome. A different genetic marker, pel, maps with ptsM, and is required for lambda DNA penetration of the cytoplasmic membrane. Earlier studies suggested that both pel function and II-BMan are encoded by the same gene, while a different gene (also in ptsM) encodes II-AMan. In the present studies, a ptsM clone, pCS13, was isolated from an E. coli HindIII gene bank in pBR322 and restored both mannose termentation and pel+ function to ptsM mutants defective in II-BMan. Subclones of pCS13 show that two distinct genes, manY and manZ, encode the pel+ function and the II-BMan protein, respectively; each gene may have its own promoter; whereas the protein encoded by manY (Pel) alone seems sufficient for lambda sensitivity, all three gene products are required for mannose fermentation, transport of the mannose analogue 2-deoxyglucose, and phosphorylation of the latter by cytoplasmic membranes. Thus, Pel is required for function of the IIMan complex. The efficiency of the complex may depend on the ratio of Pel to IIMan.