IMMUNOHISTOCHEMICAL STUDY OF CHOLINE ACETYLTRANSFERASE-IMMUNOREACTIVE PROCESSES AND CELLS INNERVATING THE PONTOMEDULLARY RETICULAR-FORMATION IN THE RAT
IMMUNOHISTOCHEMICAL STUDY OF CHOLINE ACETYLTRANSFERASE-IMMUNOREACTIVE PROCESSES AND CELLS INNERVATING THE PONTOMEDULLARY RETICULAR-FORMATION IN THE RAT
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DOI：
10.1002/cne.902950311
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发表时间：
1990-05-15
影响因子：
2.5
通讯作者：
JONES, BE
中科院分区：
文献类型：
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作者：
JONES, BE
本研究旨在探讨脑干网状结构的胆碱能神经支配，以了解胆碱能神经元在感觉-运动调节和状态控制过程中的潜在作用。应用胆碱乙酰转移酶（ChAT）银增强的过氧化物酶-抗过氧化物酶免疫组织化学方法，研究了大鼠脑桥延髓网状结构内的胆碱能细胞和突起。ChAT免疫反应阳性细胞位于脑桥中脑被盖的背侧和脚桥被盖（LDT和PPT）核内，每侧约有3，000个，分散在中线、内侧和外侧髓质网状结构中，每侧总计约有10，000个。网状结构内的胆碱能神经元通常为中等大小，并产生多个树突，这些树突在室周灰质或网状结构内延伸相当长的距离，这是其他等树突网状神经元的典型特征。一个突出的神经支配的整个pontomedullary网状结构是显而易见的静脉曲张的ChAT免疫反应阳性纤维，往往围绕大型noncholinergic网状神经元在一个典型的perisomatic模式的终止，这表明一个强有力的影响cholinergic神经支配pontomedullary网状神经元。应用辣根过氧化物酶标记的麦胚凝集素（WGA-HRP）逆行追踪结合ChAT免疫组织化学方法，研究了桥中脑胆碱能神经元对延髓内侧网状结构和脑桥外侧网状结构的支配作用。被盖背外侧核内胆碱能神经元的比例（α部）和脚桥脑被盖核在内侧髓网状结构的同侧（10 - 15%）和对侧（5 - 10%）进行逆行标记，表明对该区域的胆碱能神经支配有显着贡献，然而，也似乎部分来源于内在髓胆碱能神经元。桥中脑胆碱能神经元到达内侧延髓网状结构的主要纤维系统似乎对应于外侧被盖网状束。纤维通过这些胆碱能细胞腹侧通过脑桥被盖，在该地区的subcoeruleus，在那里他们也出现了神经支配的纤维恩通道的noncholinergic神经元的区域。一个显着比例的脑桥中脑胆碱能神经元逆行标记的外侧脑桥被盖。脑桥中脑胆碱能神经元对脑桥延髓网状结构的显著神经支配为胆碱能神经元在感觉-运动调节和状态控制，特别是异相睡眠状态中的假设作用提供了潜在的神经解剖学基础。
The present study was undertaken to examine the cholinergic innervation of the brainstem reticular formation in an effort to understand the potential role of cholinergic neurons in processes of sensory-motor modulation and state control. The cholinergic cells and processes within the pontomedullary reticular formation were studied in the rat by application of peroxidase-antiperoxidase immunohistochemistry with silver intensification for choline-acetyltransferase (ChAT). ChAT-immunoreactive cells were located in the pontomesencephalic tegmentum within the laterodorsal and pedunculopontine tegmental (LDT and PPT) nuclei, where they numbered approximately 3,000 on each side and were scattered in the midline, medial, and lateral medullary reticular formation, where they numbered approximately 10,000 in total on each side. The cholinergic neurons within the reticular formation were commonly medium in size and gave rise to multiple dendrites that extended for considerable distances within the periventricular gray or the reticular formation, as is typical of other isodendritic reticular neurons. A prominent innervation of the entire pontomedullary reticular formation was evident by varicose ChAT-immunoreactive fibers that often surrounded large noncholinergic reticular neurons in a typical perisomatic pattern of termination, suggesting a potent influence of the cholinergic innervation on pontomedullary reticular neurons. The contribution of the pontomesencephalic cholinergic neurons to the innervation of the medial medullary and lateral pontine reticular formation was studied by retrograde transport of horeseradish peroxidase conjugated wheat germ aggultinin (WGA-HRP in combination with ChAT immunohistochemistry. A proportion of the cholinergic neurons within the laterodorsal tegmental nucleus (pars alpha) and the pedunculopontine tegmental nucleus were retrogradely labelled on the ipsilateral (10-15%) and contralateral (5-10%) sides from the medial medullary reticular formation, indicating a significant contribution to the cholinergic innervation of this region, which, however, also appeared to derive in part from intrinsic medullary cholinergic neurons. The major fiber system by which the medial medullary reticular formation was reached by the pontomesencephalic cholinergic neurons appeared to correspond to the lateral tegmentoreticular tract. Fibers passed from these cholinergic cells ventrally through the lateral pontine tegmentum, in the region of the subcoeruleus, where they also appeared to innervate by fibres en passage the noncholinergic neurons of the region. A significant proportion of the pontomesencephalic cholinergic neurons were retrogradely labelled from the lateral pontine tegmentum. The prominent innervation of the pontomedullary reticular formation by the pontomesencephalic cholinergic neurons provides a potential neuroanatomical substrate for the hypothesized role of cholinergic neurons in sensory-motor modulation and state control, particularly the state of paradoxical sleep.