Adolescent exposure to nicotine modifies acute functional responses to cannabinoid agonists in rats
Adolescent exposure to nicotine modifies acute functional responses to cannabinoid agonists in rats
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DOI：
10.1016/j.bbr.2006.04.012
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发表时间：
2006-09-15
影响因子：
2.7
通讯作者：
Viveros, M. P.
中科院分区：
文献类型：
--
作者：
Marco, E. M.;Llorente, R.;Viveros, M. P.
我们研究了尼古丁和大麻素受体激动剂CP 55，940（CP）在调节雄性和雌性青春期Wistar大鼠的行为和皮质类固醇反应中的功能相互作用。对动物进行亚慢性尼古丁处理（0.4mg/kg i. p.，每天一次）。最后一次注射尼古丁后24小时，急性剂量的CP（1或100 μ g/kg i. p.）被管理。注射大麻素后30分钟，在洞板中单独测试动物，随后立即进行高架十字迷宫。我们还通过放射免疫法测定了皮质酮水平。在雄性动物中，单独给药时，CP（1 μ g/kg）和尼古丁均未诱导焦虑发生任何变化。然而，两种药物的组合导致显著的焦虑样作用。在雌性动物中，较低剂量的CP具有致焦虑作用，而本身未诱导任何作用的尼古丁可预防该反应。在洞板，亚慢性尼古丁和急性大麻素治疗相互作用的水平活动的调制和这种相互作用的性质也表现出明显的性二态性。大麻素激动剂和尼古丁都增加了皮质酮的浓度，接受这两种药物的动物比单独接受大麻素的动物表现出更高的水平。这些数据提供了证据，尼古丁和大麻素之间的功能相互作用的调制行为反应和肾上腺皮质活动在青春期大鼠的存在。(c)2006 Elsevier B. V.保留所有权利。
We have studied functional interactions between nicotine and the cannabinoid receptor agonist CP 55,940 (CP) in the modulation of behavioural and corticosterome responses of male and female adolescent Wistar rats. The animals underwent a subchronic nicotine treatment (0.4 mg/kg i.p., once daily) during the periadolescent period (postnatal days 34-43). Twenty-four hours after the last injection of nicotine an acute dose of CP (1 or 100 mu g/kg i.p.) was administered. Thirty minutes after the cannabinoid injection, the animals were tested individually in the holeboard immediately followed by the elevated plus-maze. We also measured corticosterone levels by radioimmunoassay. In males, neither CP (1 mu g/kg) nor nicotine induced any modification in anxiety when administered alone. However, the combination of the two drugs resulted in a significant anxiogenic-like effect. In females, the lower dose of CP was anxiogenic and nicotine, which did not induce any effect per se, prevented this response. In the holeboard, subchronic nicotine and the acute cannabinoid treatment interacted in the modulation of horizontal activity and the nature of this interaction also showed a clear sexual dimorphism. Both, the cannabinoid agonist and nicotine increased corticosterone concentrations and the animals receiving the two drugs showed higher levels than the animals receiving the cannabinoid alone. The data provide evidence for the existence of functional interactions between nicotine and cannabinoids in the modulation of behavioural responses and adrenocortical activity in adolescent rats. (c) 2006 Elsevier B.V. All rights reserved.