Paralytic shellfish poisoning in Haliotis tuberculata from the Galician coast:: geographical distribution, toxicity by lengths and parts of the mollusc
Paralytic shellfish poisoning in Haliotis tuberculata from the Galician coast:: geographical distribution, toxicity by lengths and parts of the mollusc
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DOI：
10.1016/s0166-445x(98)00122-2
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发表时间：
1999-07-01
影响因子：
4.5
通讯作者：
Franco, JM
中科院分区：
文献类型：
--
作者：
Bravo, I;Reyero, MI;Franco, JM
为了阐明麻痹性贝类毒素的来源，人们进行了各种研究，以描述该软体动物的面积、长度和部位的毒性分布。通过小鼠生物测定分析，加利西亚海岸不同地点的前毒性为252 +/- 25 μ g STX当量/100 g肉（μ g蛤毒素当量/100 g），高效液相色谱法（HPLC）为454 +/- 86 μ g STX当量/100 g肉。未检测到低于140 μ g STX eq/100 g肉的值。HPLC法测得的值在不同地点间无显著差异，而小鼠生物测定法测得的值有一定差异。毒素组成显示，decarbamoylsaxitoxin （dcSTX）含量最多（83-100%），而saxitoxin （STX）含量较少。不同长度组的毒素含量有显著差异，最大的长度超过85毫米，毒素含量显著增加。通过高效液相色谱法对软体动物不同部位的毒素分析显示，与肠道和软体动物的其他部位相比，足部上皮的毒素含量明显高。上皮细胞毒素含量为105 × 10(2) +/- 15 × 10(2) μ g，而肠道毒素含量为28 +/- 5 μ g，肌肉毒素含量为27 +/- 6 μ g。考虑到前者各部分毒素的重量和数量，上皮细胞携带的毒素是肌肉的2.6倍。(C) 1999 Elsevier Science B.V.版权所有
In order to clarify the origin of Paralytic Shellfish Toxins occurring in the ormers, Haliotis tuberculata, various studies were conducted to describe toxicity distribution by area, length and parts of the mollusc. Ormer toxicity from different locations of the Galician coast had a confidence interval of 252 +/- 25 mu g STX eq/100 g of meat (mu g of equivalents of saxitoxin/100 g) by mouse bioassay analyses and 454 +/- 86 mu g STX eq/100 g of meat by High Performance Liquid Chromatography (HPLC). No value of below 140 mu g STX eq/100 g of meat was detected. No significant differences were observed among locations for the values obtained by HPLC, while some differences were found by mouse bioassay. Toxin composition showed decarbamoylsaxitoxin (dcSTX) as the most abundant component (83-100%) with saxitoxin (STX) in a much smaller proportion. Significant differences were observed in the toxin content for the different length groups, toxin content increasing very significantly in the largest ormers, of over 85 mm. Toxin analyses in the different parts of the molluse by HPLC showed significantly high values for the epithelium of the foot in comparison with the gut and the rest of the mollusc. They reached 105 x 10(2) +/- 15 x 10(2) mu g of toxin/100 g of epithelium, against 28 +/- 5 mu g of toxin/100 g of gut and 27 +/- 6 mu g of toxin/100 g of muscle. Considering the weight and the quantity of toxin in each part of the ormer, the epithelium carried 2.6 times more toxin than the muscle. (C) 1999 Elsevier Science B.V. All rights reserved.