Alteration of serum inflammatory cytokines in active pulmonary tuberculosis following anti-tuberculosis drug therapy
Alteration of serum inflammatory cytokines in active pulmonary tuberculosis following anti-tuberculosis drug therapy
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DOI：
10.1016/j.molimm.2014.06.002
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发表时间：
2014-11-01
影响因子：
3.6
通讯作者：
Bahar, Bojlul
中科院分区：
文献类型：
--
作者：
Chowdhury, Imran Hussain;Ahmed, Albin Mostaque;Bahar, Bojlul
活动性肺结核(APTB)与宿主免疫系统无法控制侵袭性结核分枝杆菌(Mtb)有关。本研究的目的是定量和评价活动性肺结核患者抗结核药物治疗后血清炎性细胞因子的作用。分别于治疗前、治疗后2、4和6个月采集APTB患者和正常健康人(NHS)的血样，用细胞因子特异性ELISA法测定血清炎性细胞因子的含量。与NHS相比，APTB患者基线时血清促炎细胞因子IL-12p40(P<；0.001)、干扰素-γ(P<；0.001)、肿瘤坏死因子-α(P<；IL-1β(P<；0.001)、IL-6(P<；0.001)及抗炎细胞因子IL-10(P<；0.001)、转化生长因子-β1(P<；0.001)，而IL-4水平无明显变化。APTB患者血清中的干扰素-γ、肿瘤坏死因子-α、白介素6和转化生长因子-β1水平与细菌载量直接相关，而肿瘤坏死因子-α、白介素1-β、白介素6和转化生长因子-β1的水平与放射学严重程度有关。在基线时，NHS和APTB患者的IL-6水平差异最大，在ATD治疗后，APTB患者的IL-6水平迅速下降，并在4个月后稳定下来。结论：APTB患者ATD治疗后血清IL-6水平的轻微下降可能在机体对结核分枝杆菌感染的免疫保护中发挥重要作用，因此血清IL-6水平可以作为诊断患者治疗效果的有用指标。(C)2014爱思唯尔有限公司。保留所有权利。
Active pulmonary tuberculosis (APTB) is associated with a failure of the host immune system to control the invading Mycobacterium tuberculosis (Mtb). The objective of this study was to quantify and assess the role of serum inflammatory cytokines in active pulmonary tuberculosis patients following anti-tuberculosis drug (ATD) therapy.Blood samples were collected from APTB patients and normal healthy subjects (NHS) (total n = 204) at baseline and 2,4 and 6 months post-therapy and the abundance of serum inflammatory cytokines were measured by cytokine specific ELISA.Compared to NHS, APTB patients at baseline had higher levels of serum pro-inflammatory cytokines IL-12p40 (P < 0.001), IFN-gamma (P < 0.001), TNF-alpha (P < 0.01), IL-1 beta (P < 0.001) and IL-6 (P < 0.001) and antiinflammatory cytokines IL-10 (P < 0.001) and TGF-beta 1 (P < 0.001) while there was no change in the level of IL-4. In APTB patients, the serum levels of IFN-gamma, TNF-alpha, IL-6 and TGF-beta 1 directly relate to the bacterial load while the TNF-alpha, IL-1 beta, IL-6 and TGF-beta 1 relate to radiological severity. At baseline, the IL-6 level in NHS and APTB patients differed most and following ATD therapy, this level rapidly decreased and stabilized by 4-month in APTB patients.It is concluded that a subtle reduction in the serum level of IL-6 of the APTB patients following ATD therapy might play a vital role in immune-protection of the host against Mtb infection and hence the serum IL-6 level can be a useful marker to diagnose the effectiveness of therapy in the patients. (C) 2014 Elsevier Ltd. All rights reserved.