Electrocardiogram Recordings in Anesthetized Mice using Lead II
Electrocardiogram Recordings in Anesthetized Mice using Lead II
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DOI：
10.3791/61583
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发表时间：
2020-06-01
影响因子：
1.2
通讯作者：
Kang, Ji-One
中科院分区：
文献类型：
--
作者：
Ha, Tae Woong;Oh, Bermseok;Kang, Ji-One
心电图是评价心脏传导系统的一种有价值的工具。动物研究有助于产生关于心电图的新的遗传和药理学信息。然而，在小动物（如小鼠）体内进行心电图测量一直具有挑战性。为此，我们在麻醉小鼠中使用了一种心电图记录方法，它具有许多优点：技术上简单，价格低廉，测量时间短，即使在年轻小鼠中也是负担得起的。尽管使用麻醉的局限性，对照和实验组之间的比较可以提高灵敏度。我们用自主神经系统的激动剂和拮抗剂治疗小鼠，以确定该方案的有效性，并将我们的结果与先前的报告进行比较。我们的心电图方案检测到阿托品治疗组心率和QTc间期增加，卡巴酚治疗组心率和QTc间期降低，异丙肾上腺素治疗组心率和QTc间期升高，但未注意到普萘洛尔治疗组心电图参数有任何变化。这些结果得到了先前报告的支持，证实了该心电图方案的可靠性。因此，这种方法可以作为一种筛选方法来进行心电图测量，否则由于成本高和技术困难而不会尝试。
The electrocardiogram is a valuable tool for evaluating the cardiac conduction system. Animal research has helped generate novel genetic and pharmacological information regarding the electrocardiogram. However, making electrocardiogram measurements in small animals in vivo, such as mice, has been challenging. To this end, we used an electrocardiogram recording method in anesthetized mice with many advantages: it is a technically simple procedure, is inexpensive, has short measuring time, and is affordable, even in young mice. Despite the limitations with using anesthesia, comparisons between control and experimental groups can be performed with enhanced sensitivity. We treated mice with agonists and antagonists of the autonomic nervous system to determine the validity of this protocol and compared our results with previous reports. Our ECG protocol detected increased heart rates and QTc intervals on treatment with atropine, decreased heart rates and QTc intervals after carbachol treatment, and higher heart rates and QTc intervals with isoprenaline but did not note any change in ECG parameters on administration of propranolol. These results are supported by previous reports, confirming the reliability of this ECG protocol. Thus, this method can be used as a screening approach to making ECG measurements that otherwise would not be attempted due to high cost and technical difficulties.