Solid-state NMR spectroscopy of aligned lipid bilayers at low temperatures
Solid-state NMR spectroscopy of aligned lipid bilayers at low temperatures
复制标题
DOI：
10.1021/ja039077o
复制
发表时间：
2004-03-03
影响因子：
15
通讯作者：
Ramamoorthy, A
中科院分区：
文献类型：
--
作者：
Lee, DK;Wildman, KH;Ramamoorthy, A
这项研究首次证明，在非常低的温度（低至凝胶到液晶的相变温度）下，可以机械地排列脂质双层。在低温下对机械排列的脂质双层进行NMR实验增加了信噪比、分辨率和NMR参数的跨度。增加寿命的排列和双层样品的性质将提高固态NMR技术的应用研究膜蛋白。
This study, for the first time, demonstrates that it is possible to mechanically align lipid bilayers at a very low temperature (as low as the gel-to-liquid crystalline phase transition temperature). Performing NMR experiments on mechanically aligned lipid bilayers at a low temperature increases the signal-to-noise ratio, the resolution, and the span of NMR parameters. The increased lifetime of the alignment and the nature of the bilayer sample would enhance the application of solid-state NMR techniques to study membrane proteins.