A noncoding RNA containing a SINE-B1 motif associates with meiotic metaphase chromatin and has an indispensable function during spermatogenesis.
A noncoding RNA containing a SINE-B1 motif associates with meiotic metaphase chromatin and has an indispensable function during spermatogenesis.
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DOI：
10.1371/journal.pone.0179585
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Noce T
中科院分区：
文献类型：
--
作者：
Nakajima R;Sato T;Ogawa T;Okano H;Noce T
通过对体外小鼠胚胎干细胞生殖细胞诱导后激活的早期应答基因的研究，我们分离出了一种长链非编码RNA，该RNA包含一个短穿插元件(sin)-B1F基序，命名为R53。原位杂交和northern blot分析显示，R53亚片段RNA带有一个B1F基序，由初级转录本加工而成，在成年睾丸中表达，主要定位于精子发生过程中的减数分裂中期染色质中。最近对染色体相关rna的研究探索了非编码rna的新功能。具体而言，染色体结合的非编码rna不仅是染色体的结构成分，而且是招募表观遗传调节剂进行转录调控的支架，它们在细胞周期中动态重排。然而，很少有研究探索减数分裂染色质；因此，R53 RNA似乎是精子发生过程中第一个与中期染色质紧密相关的长链非编码RNA。此外，使用慢病毒介导的RNAi将R53敲低注入小鼠睾丸和器官培养，发现减数分裂后细胞显著减少，数个减数分裂后基因不规则上调，这表明sin - b1衍生的非编码RNA R53可能在关键精子发生基因的转录调控中起着不可或缺的作用。
A search for early response genes that are activated following germ cell induction from mouse embryonic stem cells in vitro led us to the isolation of a long noncoding RNA that contains a SINE (short interspersed element)-B1F motif that was named R53. In situ hybridization and northern blot analyses revealed that the R53 subfragment RNA bears a B1F motif, is processed from the primary transcript, is expressed in adult testis and is predominantly localized in meiotic metaphase chromatin during spermatogenesis. Recent studies of chromosome-associated RNAs have explored novel functions of noncoding RNAs. Specifically, chromosome-bound noncoding RNAs function not only as structural components of chromosome but also as scaffolds that recruit epigenetic modulators for transcriptional regulation, and they are dynamically rearranged during the cell cycle. However, few studies have explored meiotic chromatin; thus, R53 RNA appears to be the first long noncoding RNA to be tightly associated with the metaphase chromatin during spermatogenesis. Furthermore, R53 knockdown using a lentivirus-mediated RNAi injected into mouse testis and organ culture of the fragments revealed a remarkable reduction in postmeiotic cells and irregular up-regulation of several postmeiotic genes, which suggests the possibility that the SINE-B1-derived noncoding RNA R53 plays an indispensable role in the transcriptional regulation of key spermatogenesis genes.