METABOLIC CHARACTERISTICS OF HUMAN ADIPOSE-TISSUE INVIVO
METABOLIC CHARACTERISTICS OF HUMAN ADIPOSE-TISSUE INVIVO
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DOI：
10.1042/cs0760509
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发表时间：
1989-05-01
期刊：
影响因子：
6
通讯作者：
WHYTE, PL
中科院分区：
文献类型：
--
作者：
FRAYN, KN;COPPACK, SW;WHYTE, PL
提出了一种从前腹壁皮下脂肪组织中抽取静脉引流液的方法。在许多学科中，这是一个很大的仓库，似乎很适合这样的研究，因为它被外斜肌的腱膜完全与下面肌肉的静脉引流分开。8名正常受试者禁食一夜，并在摄入75 g葡萄糖后进行120分钟的研究。腹壁引流液中底物浓度与动脉血和前臂肌肉引流液中底物浓度比较。禁食过夜后，腹壁引流液中非酯化脂肪酸和甘油浓度高（动脉水平的3 - 4倍）。葡萄糖摄入后，动脉和腹腔静脉水平下降，动静脉差异缩小。前臂在禁食时显示非酯化脂肪酸的摄取，但在葡萄糖摄入后没有，任何时候甘油的动静脉差异都不显著。腹壁组织在禁食期间葡萄糖摄取表现出微小的动静脉差异，在葡萄糖摄入后增加。尽管整个过程中都产生了乳酸，但其与葡萄糖摄取的摩尔比低于其他表面部位的报道，这表明皮肤代谢的贡献很小。葡萄糖负荷后，前臂肌肉的葡萄糖摄取动静脉差异增加幅度更大，时间更长，但乳酸的释放或摄取不一致。我们得出结论，用这种技术研究的组织主要是脂肪。该技术在研究人体体重调节的代谢基础方面具有广泛的应用前景。
A method was developed for sampling the venous drainage from the subcutaneous adipose tissue of the anterior abdominal wall. This is a large depot in many subjects, and seems well suited to such studies as it is completely separated from the venous drainage of the underlying muscle by the aponeurosis of the external oblique muscle. Eight nomal subjects were studied after an overnight fast, and for 120 min after ingestion of 75 g of glucose. Concentrations of substrates in the abdominal wall drainage were compared with those in arterialized blood and in forearm muscle drainage. Non-esterified fatty acid and glycerol concentrations in the abdominal wall drainage were high (three four times to the arterial level) after overnight fast. After glucose ingestion, arterial and abdominal venous levels fell and the arteriovenous differences narrowed. The forearm showed uptake of non-esterified fatty acids when fasting but not after glucose ingestion, with no significant arteriovenous difference for glycerol at any time. The abdominal wall tissues showed a small arteriovenous difference for glucose uptake during fasting, which increased after glucose ingestion. Although lactate was produced throughout, its molar ratio to glucose uptake was less than that reported for other superfical sites, suggesting only a minor contribution of skin metabolism. Forearm muscle showed a larger and more prolonged increase in arteriovenous difference for glucose uptake after the glucose load, but no consistent release or uptake of lactate. We conclude that the tissue studied by this technique is predominantly adipose. This technique may have wide application in studies of the metabolic basis for body weight regulation in man.