Untargeted Metabolite Profiling of Adipose Tissue in Hyperlipidemia Rats Exposed to Hawthorn Ethanol Extracts
Untargeted Metabolite Profiling of Adipose Tissue in Hyperlipidemia Rats Exposed to Hawthorn Ethanol Extracts
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DOI：
10.1111/1750-3841.14491
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发表时间：
2019-04-01
影响因子：
3.9
通讯作者：
Sun, Baoguo
中科院分区：
文献类型：
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作者：
Hu, Chuanqin;Zhang, Yu;Sun, Baoguo
采用高通量代谢组学方法研究山楂乙醇提取物（HEE）对高脂血症大鼠脂肪组织代谢的影响。HPLC分析表明，HEE主要由绿原酸、金丝桃苷、异槲皮苷、芦丁、牡荆素、槲皮素和芹菜素组成。与高脂饮食模型相比，HEE给药显著降低了总胆固醇、甘油三酯和低密度脂蛋白胆固醇的水平。采用气相色谱-质谱法鉴定脂肪代谢物谱。许多内源性分子被高脂饮食改变，并在HEE干预后恢复。在食用HEE后，检测到脂肪中的代谢产物，包括L-苏氨酸、天冬氨酸、谷氨酰胺、甘露糖、肌醇和油酸。15种代谢产物被鉴定为高脂血症的潜在生物标志物。途径分析表明，判别代谢产物主要包括脂肪酸生物合成、半乳糖代谢、不饱和脂肪酸生物合成、精氨酸和脯氨酸代谢、丙氨酸、天冬氨酸和谷氨酸代谢、甘油脂代谢和类固醇生物合成。这些代谢产物和代谢网络的发现为探讨山楂乙醇提取物对大鼠脂肪组织降脂作用的分子机制提供了新的思路。实际应用在中国，高脂血症的比例很高。山楂因其健康益处、低毒、有效且可能适合长期使用而引起越来越多的关注。
The study aimed to explore the metabolic changes of adipose tissue of hyperlipidemia rats with hawthorn ethanol extracts (HEE) consumption by a high-throughput metabolomics approach. HEE were mainly composed of chlorogenic acid, hyperoside, isoquercitrin, rutin, vitexin, quercetin, and apigenin by HPLC analysis. HEE administration significantly lowered levels of the total cholesterols, triglyceride and low-density lipoprotein cholesterol as compared to the high-fat diet model. Gas chromatography-mass spectrometry was used to identify adipose metabolite profiles. Numerous endogenous molecules were altered by high-fat diet and restored following intervention of HEE. Metabolites elevated in adipose, including l-threonine, aspartic acid, glutamine, mannose, inositol and oleic acid, were detected after HEE consumption. Fifteen metabolites were identified as potential biomarkers of hyperlipidemia. Pathway analysis showed that most of the discriminant metabolites were included in fatty acid biosynthesis, galactose metabolism, biosynthesis of unsaturated fatty acids, arginine and proline metabolism, alanine, aspartate and glutamate metabolism, glycerolipid metabolism and steroid biosynthesis. These metabolites and metabolic networks we found offer new insights into exploring the molecular mechanisms of lipid-lowering of hawthorn ethanol extracts on adipose tissue of rats. Practical Application There was a very high proportion of hyperlipidemia in China. Hawthorn is attracting increasing attention owing to their health benefits, low toxicity, effectiveness and might be suitable for long-term use.