THE GENOMIC INSTABILITY OF YEAST CDC6-1/CDC6-1 MUTANTS INVOLVES CHROMOSOME STRUCTURE AND RECOMBINATION
THE GENOMIC INSTABILITY OF YEAST CDC6-1/CDC6-1 MUTANTS INVOLVES CHROMOSOME STRUCTURE AND RECOMBINATION
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DOI：
10.1007/bf00290230
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发表时间：
1995-11-01
期刊：
影响因子：
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通讯作者：
ESPOSITO, MS
中科院分区：
文献类型：
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作者：
BRUSCHI, CV;MCMILLAN, JN;ESPOSITO, MS
当温度敏感性细胞分裂周期突变cdc 6 -1纯合的酿酒酵母二倍体细胞在半容许温度下生长时，它们表现出升高的基因组不稳定性，如增强的有丝分裂基因转换、有丝分裂基因间重组、染色体丢失、染色体获得和染色体重排所示。采用横向交变场凝胶电泳（TAFE）分离的染色体的定量Southern分析，我们已经证明，2N-1细胞单体的染色体VII，由于cnc 6 -1缺陷，表现出缓慢的增长，随后产生2N的变体，以正常的增长率与恢复染色体VII的二体性。TAFE凝胶分析还表明，cdc 6 -1/cdc 6 -1二倍体产生新长度的异常染色体。我们提出了一个解释的cdc 6 -1突变，这表明，超重组，染色体丢失，染色体增益和染色体重排，反映异常有丝分裂cdc 6 -1/cdc 6 -1细胞含有染色体没有完全复制诱导的基因组不稳定性。
When diploid cells of Saccharomyces cerevisiae homozygous for the temperature-sensitive cell division cycle mutation cdc6-1 are grown at a semipermissive temperature they exhibit elevated genomic instability, as indicated by enhanced mitotic gene conversion, mitotic intergenic recombination, chromosomal loss, chromosomal gain, and chromosomal rearrangements. Employing quantitative Southern analysis of chromosomes separated by transverse alternating field gel electrophoresis (TAFE), we have demonstrated that 2N-1 cells monosomic for chromosome VII, owing to the cnc6-1 defect, show slow growth and subsequently yield 2N variants that grow at a normal rate in association with restitution of disomy for chromosome VII. Analysis of TAFE gels also demonstrates that cdc6-1/cdc6-1 diploids give rise to aberrant chromosomes of novel lengths. We propose an explanation for the genomic instability induced by the cdc6-1 mutation, which suggests that hyper-recombination, chromosomal loss, chromosomal gain and chromosomal rearrangements reflect aberrant mitotic division by cdc6-1/cdc6-1 cells containing chromosomes that have not replicated fully.