Water‐insoluble fraction of airborne particulate matter (PM10) induces oxidative stress in human lung epithelial A549 cells
Water‐insoluble fraction of airborne particulate matter (PM10) induces oxidative stress in human lung epithelial A549 cells
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DOI：
10.1002/tox.21750
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发表时间：
2014-02
影响因子：
4.5
通讯作者：
Shuo Yi;Fang Zhang;Fang Qu;Wenjun Ding
中科院分区：
文献类型：
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作者：
Shuo Yi;Fang Zhang;Fang Qu;Wenjun Ding
暴露在空气动力学直径小于10μm的环境空气中的颗粒物(PM10)会危害公共健康，并增加患肺癌和其他疾病的风险。最近的研究表明，氧化应激是暴露在PM10下的毒性效应的一个关键机制。在体外研究中，已知PM10的几个水溶部分(SPM10)能够诱导氧化应激。在这项研究中，我们研究了PM10的水不溶部分(IPM10)是否也能够诱导氧化应激和氧化损伤。用10μg/mLsPM10、iPM10或总PM10(TPM10)处理人肺上皮细胞A549 24 h，观察到3种PM10制剂均可降低A549细胞的存活率，并诱导细胞死亡。我们进一步发现，与sPM10和tPM10暴露相似，iPM10暴露的细胞内过氧化氢(H_2O_2)水平显著增加，同时过氧化氢酶活性显著低于未暴露的对照细胞，从而导致明显的DNA损伤。我们通过电感耦合等离子体质谱(ICPMS)分析获得的数据表明，铁是所有三种PM10制剂中含量最丰富的金属。因此，我们已经证明，与sPM10类似，iPM10也能够诱导氧化应激，可能是通过诱导过氧化氢的产生和削弱酶的抗氧化防御，通过铁催化的Fenton反应导致DNA氧化损伤甚至细胞死亡。2012Wiley期刊，Inc.环境毒物29：226-233,2014。
Exposure to ambient airborne particulate matter (PM) with an aerodynamic diameter less than 10 μm (PM10) links with public health hazards and increases risk for lung cancer and other diseases. Recent studies have suggested that oxidative stress is a key mechanism underlying the toxic effects of exposure to PM10. Several components of water‐soluble fraction of PM10 (sPM10) have been known to be capable of inducing oxidative stress in in vitro studies. In this study, we investigated if water‐insoluble fraction of PM10 (iPM10) could be also capable of inducing oxidative stress and oxidative damage. Human lung epithelial A549 cells were exposed to 10 μg/mL of sPM10, iPM10 or total PM10 (tPM10) preparation for 24 h. Here, we observed that all three PM10 preparations reduced cell viability and induced apoptotic cell death in A549 cells. We further found that, similar to the exposure to sPM10 and tPM10, the intracellular level of hydrogen peroxide (H2O2) in the iPM10‐exposed cells was increased significantly; meanwhile the activity of catalase was decreased significantly as compared with the unexposed control cells, resulting in significant DNA damage. Our data obtained from inductively coupled plasma‐mass spectrometry (ICP‐MS) assays showed that iron is the most abundant metal in all three PM10 preparations. Thus, we have demonstrated that, similar to sPM10, iPM10 is also capable of inducing oxidative stress by probably inducing generation of H2O2 and impairing enzymatic antioxidant defense, resulting in oxidative DNA damage and even apoptotic cell death through the iron‐catalyzed Fenton reaction. © 2012 Wiley Periodicals, Inc. Environ Toxicol 29: 226–233, 2014.