ATP CONTENT OF HUMAN OOCYTES AND DEVELOPMENTAL POTENTIAL AND OUTCOME AFTER IN-VITRO FERTILIZATION AND EMBRYO-TRANSFER
ATP CONTENT OF HUMAN OOCYTES AND DEVELOPMENTAL POTENTIAL AND OUTCOME AFTER IN-VITRO FERTILIZATION AND EMBRYO-TRANSFER
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DOI：
10.1093/oxfordjournals.humrep.a135954
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发表时间：
1995-02-01
影响因子：
6.1
通讯作者：
LEE, J
中科院分区：
文献类型：
--
作者：
VANBLERKOM, J;DAVIS, PW;LEE, J
对20例年龄、生育史、卵巢刺激方案、卵母细胞质量、子宫移植胚胎数量、分期和形态等方面相匹配的非男性因素体外受精（IVF）患者，研究了成熟卵母细胞ATP含量与同胞胚胎子宫移植后发育潜能的关系。ATP含量测定未受精和未受精的卵母细胞为每个队列，并为胚胎发育后，dispermic受精或显着碎片。在用线粒体氧化磷酸化解偶联剂处理的培养小鼠卵母细胞中，研究了ATP含量降低对减数分裂成熟、受精和植入前发育的影响。结果表明，小鼠和人卵母细胞在广泛的ATP含量范围内均发生减数分裂成熟，并且正常外观的中期II人卵母细胞的ATP含量在队列之间可能存在显著差异;然而，人类中继续胚胎发生和植入的更高潜力与从ATP含量大于或等于2 pmol/卵母细胞的卵母细胞队列发育的胚胎相关。研究结果进行了讨论，就可能的病因和不同的卵母细胞ATP含量的胚胎发育的发育后果，在何种程度上线粒体功能可能会决定或影响继续发育能力的胚胎出现正常和发育可行的早期卵裂阶段。
The relationship between the ATP content of mature human oocytes and developmental potential after uterine transfer of sibling embryos was examined in 20 non-male factor invitro fertilization (IVF) patients matched for age, fertility history, ovarian stimulation protocol, oocyte quality and number, stage and morphology of embryos at uterine transfer. ATP content was determined for uninseminated and unfertilized oocytes for each cohort, and for embryos that developed after dispermic fertilization or which showed significant fragmentation. The effect of reduced ATP content on meiotic maturation, fertilization and preimplantation development was examined in cultured mouse oocytes treated with uncouplers of mitochondrial oxidative phosphorylation. The results demonstrated that meiotic maturation occurs in both mouse and human oocytes over a wide range of ATP contents, and that the ATP content of normal-appearing, metaphase II human oocytes can differ significantly between cohorts; however, a higher potential for continued embryogenesis and implantation in the human is associated with embryos that develop from cohorts of oocytes with ATP contents greater than or equal to 2 pmol/oocyte. The findings are discussed with respect to possible aetiologies and developmental consequences for embryonic development of different oocyte ATP contents, and the extent to which mitochondrial function may determine or influence the continued developmental capacity of embryos which appear normal and developmentally viable at the early cleavage stages.