Adhesion molecules during somitogenesis in the avian embryo.
Adhesion molecules during somitogenesis in the avian embryo.
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DOI：
10.1083/jcb.104.5.1361
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发表时间：
1987-05
期刊：
影响因子：
--
通讯作者：
Thiery JP
中科院分区：
文献类型：
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作者：
Duband JL;Dufour S;Hatta K;Takeichi M;Edelman GM;Thiery JP
在鸟类胚胎中，有些体构成了异聚模式的形态单位。有些细胞是由间质、节段板形成的上皮，随后重组为皮组、肌组和硬膜组。在这项研究中，我们以躯体发生为基础来研究早期脊椎动物形态发生过程中的组织重塑。特别强调了粘附促进分子，神经细胞粘附分子（N-CAM）， n-钙粘蛋白，纤维连接蛋白和层粘连蛋白的分布和可能的互补作用。在体外，节段板细胞和体细胞均表现出钙依赖性和钙非依赖性的细胞聚集系统，分别可被抗n -cadherin和抗n - cam抗体抑制。在体内，N-cadherin的时空表达与体体的形成和局部破坏密切相关。相反，N-CAM患病率的变化并不严格伴随着躯体上皮向真皮组织和硬膜组织的重塑。还观察到，在cam介导的接触被调节后，纤维连接蛋白和层粘连蛋白在细胞外空间中二级重组。在体体体外培养系统中，n -钙粘蛋白在体体外植体释放的单个细胞上丢失，当这些细胞达到融合并建立细胞间接触时重新表达。在体外组织解离实验中，针对n -钙粘蛋白或无钙培养基的抗体强烈且可逆地解离节段板和体的外植体。针对N-CAM的抗体仅对节段平板外植体具有较小的破坏作用。相反，纤维连接蛋白和层粘连蛋白抗体不会干扰外植体内细胞的内聚。这些结果强调了CAMs在一些瞬态胚胎上皮形成和重塑过程中可能的细胞表面调节作用。这表明N-cadherin在组织重塑的控制中起主要作用，N-CAM也参与了这一过程，但程度较轻。基质粘附分子，纤维连接蛋白和层粘连蛋白，似乎在这些过程的调节中并不起主要作用，但可能参与细胞定位和上皮结构的稳定。
In avian embryos, somites constitute the morphological unit of the metameric pattern. Somites are epithelia formed from a mesenchyme, the segmental plate, and are subsequently reorganized into dermatome, myotome, and sclerotome. In this study, we used somitogenesis as a basis to examine tissue remodeling during early vertebrate morphogenesis. Particular emphasis was put on the distribution and possible complementary roles of adhesion-promoting molecules, neural cell adhesion molecule (N-CAM), N-cadherin, fibronectin, and laminin. Both segmental plate and somitic cells exhibited in vitro calcium- dependent and calcium-independent systems of cell aggregation that could be inhibited respectively by anti-N-cadherin and anti-N-CAM antibodies. In vivo, the spatio-temporal expression of N-cadherin was closely associated with both the formation and local disruption of the somites. In contrast, changes in the prevalence of N-CAM did not strictly accompany the remodeling of the somitic epithelium into dermamyotome and sclerotome. It was also observed that fibronectin and laminin were reorganized secondarily in the extracellular spaces after CAM-mediated contacts were modulated. In an in vitro culture system of somites, N-cadherin was lost on individual cells released from somite explants and was reexpressed when these cells reached confluence and established intercellular contacts. In an assay of tissue dissociation in vitro, antibodies to N-cadherin or medium devoid of calcium strongly and reversibly dissociated explants of segmental plates and somites. Antibodies to N-CAM exhibited a smaller disrupting effect only on segmental plate explants. In contrast, antibodies to fibronectin and laminin did not perturb the cohesion of cells within the explants. These results emphasize the possible role of cell surface modulation of CAMs during the formation and remodeling of some transient embryonic epithelia. It is suggested that N-cadherin plays a major role in the control of tissue remodeling, a process in which N-CAM is also involved but to a lesser extent. The substratum adhesion molecules, fibronectin and laminin, do not appear to play a primary role in the regulation of these processes but may participate in cell positioning and in the stabilization of the epithelial structures.