Effect of acute exposure to ammonia and BFT alterations on Rhynchocypris lagowski: Digestive enzyme, inflammation response, oxidative stress and immunological parameters.
Effect of acute exposure to ammonia and BFT alterations on Rhynchocypris lagowski: Digestive enzyme, inflammation response, oxidative stress and immunological parameters.
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DOI：
10.1016/j.etap.2020.103380
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发表时间：
2020-05
影响因子：
4.3
通讯作者：
Zhe Yu;Xuexiang Wu;Lu-Juan Zheng;Zhong-Yi Dai;Li‐fang Wu
中科院分区：
文献类型：
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作者：
Zhe Yu;Xuexiang Wu;Lu-Juan Zheng;Zhong-Yi Dai;Li‐fang Wu
生物絮体技术是一种新型的绿色养殖技术，它不仅能消除水中氮的残留，还能提高水生动物的免疫力和抗氧化能力。采用56 d饲养试验和96 h氨激发试验，研究了急性氨暴露和BFT改变对人工养殖的人工根据C/N比的不同，试验分为C/N 10.8：1（对照组）、C/N 15：1、C/N 20：1和C/N 25：1四个组。结果表明，与对照组相比，C/N20组的增重率（WGR）、特定生长率（SGR）和蛋白质效率比（PER）显著提高，而食物转化率（FCR）显著降低（P< 0.05）。消化酶淀粉酶（AMS）、脂肪酶（LPS）、蛋白酶（PRS）、纤维素酶（CES）活性，免疫酶补体C3、补体C4、一氧化氮合酶（NOS）、免疫球蛋白M（IgM）活性，血清生化溶菌酶（LSZ）、谷丙转氨酶（GPT）、谷草转氨酶（GOT）、碱性磷酸酶（AKP）活性显著升高;炎症因子TNF-α、IL-1β、IL-2、IL-6。C/N20组经56天饲养试验和攻毒试验后，出现了兔尾畸形（P< 0.05）。比较R.结果表明，C/N20组幼鱼的超氧化物歧化酶（SOD）活性、总抗氧化能力（T-AOC）、过氧化氢酶（CAT）活性和谷胱甘肽还原酶（GR）活性显著高于对照组（P< 0.05），丙二醛（MDA）含量显著低于对照组（P< 0.05）。总体而言，这些发现表明BFT不仅可以增强R。提高兔肉生长性能、消化酶活性、增强抗氧化状态，还能提升免疫反应，提高对氨胁迫的抵抗能力。
Biofloc technology (BFT) is a new green culture technology that is intended not merely to eradicate nitrogenous residues but also enhance immunity and antioxidant activity in aquatic animals. A 56-day feeding trial and a 96 h ammonia challenge test were implemented to evaluate the effect of acute exposure to ammonia and BFT alterations onRhynchocypris lagowski: digestive enzyme, inflammation response, oxidative stress and immunological parameters in zero water exchange tanks. According to the differences of C/N ratios, the experiment was divided into four groups: C/N 10.8:1 (control group), C/N 15:1, C/N 20: 1 and C/N 25:1. The results provided evidence that weight gain rate (WGR), specific growth rate (SGR) and protein efficiency ratio (PER) were significantly elevated in C/N 20, while food conversion rate (FCR) was considerably decreased (P< 0.05) compared to the control. Significant increases in digestive enzyme amylase (AMS), lipase (LPS), protease (PRS) and cellulase (CES) activity; Immune enzyme complement C3, complement C4, Nitric Oxide Synthase (NOS) and immunoglobulin M (IgM) activity; Serum biochemical lysozyme (LSZ), glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT) and alkaline phosphatase (AKP) activity; Inflammation TNF-α, IL-1β, IL-2, IL-6 ofR. lagowskiswere found in the C/N 20 group after a 56-day feeding trial and a challenging trial (P< 0.05). Comparing the antioxidant capacity ofR. lagowskiin gills, brains and spleen of juveniles from the four experimental groups, the activity of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT) activity and glutathion reductases (GR) activity of juveniles in the C/N 20 group were significant higher (P< 0.05), and the content of malondialdehyde (MDA) was considerably lower than that in the control. Overall, these findings suggest that BFT not only can enhanceR. lagowskigrowth performance, digestive enzyme activity, and strengthen antioxidant status but also upgrade immune response, improve the ability of resistance to ammonia stress.