Temperature-Dependent Dynamics of Giant Vesicles Composed of Hydrolysable Lipids Having an Amide Linkage
Temperature-Dependent Dynamics of Giant Vesicles Composed of Hydrolysable Lipids Having an Amide Linkage
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由具有酰胺键的可水解脂质组成的巨型囊泡的温度依赖性动力学
DOI：
10.1021/acs.langmuir.9b02707
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发表时间：
2019
期刊：
影响因子：
3.9
通讯作者：
Banno Taisuke
中科院分区：
文献类型：
--
作者：
Tameyuki Maito;Hiranaka Hisato;Toyota Taro;Asakura Kouichi;Banno Taisuke
人们设计和合成了各种两亲性化合物，包括表面活性剂和脂类，以改进和创造新的功能。尤其是由合成脂质组成的巨大囊泡(GV)类细胞行为的出现，在细胞化学模型的发展中引起了极大的关注。这项研究的目的是测量具有酰胺键的可水解性阳离子脂类在裂解和后续生长过程中诱导的GV形态随温度的变化。差示扫描量热仪、环境响应探针的荧光光谱和共聚焦拉曼显微镜的结果表明，所观察到的动力学是由于热刺激引起的囊泡膜的变化，包括脂质成分的变化。
Various amphiphiles including surfactants and lipids have been designed and synthesized to improve and create new functionalities. In particular, the emergence of cell-like behaviors of giant vesicles (GVs) composed of synthetic lipids has drawn much attention in the development of chemical models for cells. The aim of this study was to measure temperature-dependent morphological changes of GVs induced by fragmentation and subsequent growth using hydrolysable cationic lipids having an amide linkage. Results from differential scanning calorimetry, fluorescence spectroscopy using an environment-responsive probe, and confocal Raman microscopy showed that the dynamics observed were due to changes in the vesicle membrane, including variation in the lipid composition, induced by thermal stimulation.