Introduction of a Na+/H+ antiporter gene from Atriplex gmelini confers salt tolerance to rice
Introduction of a Na+/H+ antiporter gene from Atriplex gmelini confers salt tolerance to rice
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DOI：
10.1016/s0014-5793(02)03679-7
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发表时间：
2002-12-18
期刊：
影响因子：
3.5
通讯作者：
Hayakawa, T
中科院分区：
文献类型：
--
作者：
Ohta, M;Hayashi, Y;Hayakawa, T
我们设计了一个盐敏感水稻品种（Oryza sativa cv）。Kinuhikari)表达来自盐生植物Atriplex gmelini （AgNHX1）的液泡型Na+/H+反转运基因。转基因水稻液泡型Na+/H+反转运蛋白的活性是野生型水稻的8倍。非胁迫处理后的耐盐性试验表明，过表达AgNHX1的转基因植株可以在300 mM NaCl条件下存活3天，而野生型植株则不能。上述结果表明，过表达Na+/H+反转运基因可显著提高水稻耐盐性。(C) 2002年欧洲生化学会联合会。Elsevier Science B.V.版权所有。
We engineered a salt-sensitive rice cultivar (Oryza sativa cv. Kinuhikari) to express a vacuolar-type Na+/H+ antiporter gene from a halophytic plant, Atriplex gmelini (AgNHX1). The activity of the vacuolar-type Na+/H+ antiporter in the transgenic rice plants was eight-fold higher than that in wild-type rice plants. Salt tolerance assays followed by nonstress treatments showed that the transgenic plants overexpressing AgNHX1 could survive under conditions of 300 mM NaCl for 3 days while the wild-type rice plants could not. These results indicate that overexpression of the Na+/H+ antiporter gene in rice plants significantly improves their salt tolerance. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.