Facilitated production of secretory IgA against Shiga toxin B subunits by intranasal application of antigen-coated polystyrene microspheres
Facilitated production of secretory IgA against Shiga toxin B subunits by intranasal application of antigen-coated polystyrene microspheres
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DOI：
10.1111/j.1348-0421.2005.tb03714.x
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发表时间：
2005-01-01
影响因子：
2.6
通讯作者：
Imai, Y
中科院分区：
文献类型：
--
作者：
Kurohane, K;Kobayashi, C;Imai, Y
我们研究了微球作为抗原载体在粘膜免疫中的作用。将志贺毒素B亚基（Stx1B）吸附在6μm聚苯乙烯微球上，然后与霍乱毒素（CT）一起给小鼠鼻内给药。使用微球增强了局部粘膜部位 Stx1B 特异性血清 IgG 的产生和分泌性 IgA 的产生。当使用 OVA 作为模型抗原时，微球的使用增强了分泌型 IgA 的产生，但血清 IgG 的产生没有增强。这些结果表明，微球提供了一种增强针对 Stx1B 的免疫反应的有效手段，且免疫原性较弱。
We examined the effects of microspheres as antigen carriers in mucosal immunization. Shiga toxin B subunits (Stx1B) were adsorbed on 6 mum polystyrene microspheres, which were then intranasally administered to mice together with cholera toxin (CT). Stx1B-specific serum IgG production and secretory IgA production at local mucosal sites were enhanced by the use of microspheres. When OVA was used as a model antigen, secretory IgA production but not serum IgG production was enhanced on the use of microspheres. These results indicated that microspheres provide a useful means of potentiating the immune response against Stx1B with weak immunogenicity.