Anti-myocardial ischaemic effect of pseudoginsenoside F11 by inhibiting expression of beta1-adrenoceptor in rats with coronary artery ligation
Anti-myocardial ischaemic effect of pseudoginsenoside F11 by inhibiting expression of beta1-adrenoceptor in rats with coronary artery ligation
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DOI：
10.1016/j.jff.2017.06.053
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发表时间：
2017-09-01
影响因子：
5.6
通讯作者：
Liu, Jin-Ping
中科院分区：
文献类型：
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作者：
Zhang, Nan-Qi;Wang, Cui-Zhu;Liu, Jin-Ping
采用冠状动脉结扎大鼠模型，观察了西洋参的特征性成分三七皂甙F_（11）（PF_（11））的抗心肌缺血作用。实验数据和组织病理学检查均表明，灌胃给予6和12 mg/kg剂量的PF 11可显著改善心功能和缩小梗死面积。心肌组织中肌酸激酶（CK）、乳酸脱氢酶（LDH）、天冬氨酸转氨酶（AST）、丙二醛（MDA）、心肌肌钙蛋白T（cTnT）水平降低，超氧化物歧化酶（SOD）活性升高。流式细胞术和Western blotting检测结果显示，PF 11可降低β 1-肾上腺素能受体（β 1-AR）的表达。此外，用于分子对接的Glide XP软件（Schrodinger）解开了PF 11和β 1-AR之间的可能结合位点。本研究为PF 11的抗心肌缺血作用提供了充分的证据，提示PF 11可能是一种潜在的治疗心肌缺血损伤的膳食补充剂。(C)2017爱思唯尔有限公司版权所有
The anti-myocardial ischaemic effect of pseudoginsenoside F11 (PF11), a characteristic constituent of American ginseng, was evaluated in rats with coronary artery ligation. Both data and histopathological examination demonstrated that intragastric administration of PF11 at doses of 6 and 12 mg/kg could significantly improve heart function and decrease infarct size. Meanwhile, the levels of creatine kinase (CK), lactate dehydrogenase (LDH), aspartate transaminase (AST), malondialdehyde (MDA), and cardiac troponin T (cTnT) decreased, while the activity of superoxide dismutase (SOD) increased. Flow cytometry and western blotting were used to explore the underlying mechanism, and results showed that the expression of beta1-adrenoceptor (beta 1-AR) was decreased by PF11. Furthermore, Glide XP software (Schrodinger) for molecular docking unravelled the probable binding sites between PF11 and beta 1-AR. This study provides substantial evidence for the effect of PF11 on myocardial ischaemia, and indicates that PF11 might be a potential dietary supplement for treatment of myocardial ischaemia injury. (C) 2017 Elsevier Ltd. All rights reserved.