In vivo synthesis of europium selenide nanoparticles and related cytotoxicity evaluation of human cells
In vivo synthesis of europium selenide nanoparticles and related cytotoxicity evaluation of human cells
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DOI：
10.1016/j.enzmictec.2016.08.012
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发表时间：
2016-12-01
影响因子：
3.4
通讯作者：
Park, Tae Jung
中科院分区：
文献类型：
--
作者：
Kim, Eun Bee;Seo, Ji Min;Park, Tae Jung
纳米技术致力于结合新材料开发贵重纳米颗粒。由于纳米粒子因其组成而表现出独特的光学、电学和磁学性质，因此开发安全、经济、环保的合成技术已成为一个重要的课题。本研究利用表达重金属结合蛋白、植物络合蛋白合成酶和金属硫蛋白的重组大肠杆菌细胞，在体内合成了硒化铕(EuSe)纳米颗粒。通过紫外-可见光谱、荧光光谱、X射线衍射、能量色散X射线和透射电子显微镜等手段证实了EuSe纳米粒子的形成。合成的EuSe纳米粒子具有较高的荧光强度和较强的磁性。此外，还研究了EuSe纳米粒子对癌细胞株的抗癌作用。这种生物合成纳米粒子的策略由于其简单和无毒，在生物医学领域作为生物成像工具和药物载体具有很大的潜力。(C)2016 Elsevier Inc.保留所有权利。
Nanotechnology strives to combine new materials for development of noble nanoparticles. As the nanoparticles exhibit unique optical, electronic, and magnetic properties depending on their composition, developing safe, cost-effective and environmentally friendly technologies for the synthesis have become an important issue. In this study, in vivo synthesis of europium selenide (EuSe) nanoparticles was performed using recombinant Escherichia coli cells expressing heavy-metal binding proteins, phytochelatin synthase and metallothionein. The formation of EuSe nanoparticles was confirmed by using UV-vis spectroscopy, spectrofluorometry, X-ray diffraction, energy dispersive X-ray and transmission electron microscopy. The synthesized EuSe nanoparticles exhibited high fluorescence intensities as well as strong magnetic properties. Furthermore, anti-cancer effect of EuSe nanoparticles against cancer cell lines was investigated. This strategy for the biogenic synthesis of nanoparticles has a great potential as bioimaging tools and drug carrying agents in biomedical fields due to its simplicity and nontoxicity. (C) 2016 Elsevier Inc. All rights reserved.