Pollen-Stigma Adhesion in Kale Is Not Dependent on the Self-(In)Compatibility Genotype
Pollen-Stigma Adhesion in Kale Is Not Dependent on the Self-(In)Compatibility Genotype
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DOI：
10.1104/pp.115.3.1221
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发表时间：
1997-11
期刊：
影响因子：
--
通讯作者：
Doan-Trung Luu;P. Heizmann;C. Dumas
中科院分区：
文献类型：
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作者：
Doan-Trung Luu;P. Heizmann;C. Dumas
花粉在开花植物柱头上的粘附是被子植物成功繁殖的关键步骤，长期以来被认为在自交不亲和性方面表现出一定的特异性。我们利用浓蔗糖溶液（50%，w/v）施加的阿基米德浮力释放固定在柱头表面的花粉粒，对羽衣甘蓝（Brassica oleracea）的花粉柱头粘附力（以牛顿表示）进行了定量测量。我们证明，花粉粘附随用作伴侣的植物的基因型而变化，但在所有情况下，在授粉后约 30 至 60 分钟内，花粉粘附随着时间的推移而增加。与授粉的自花或异花授粉状态无关，也与亲本的自交亲和或不亲和基因型无关。只有在花粉管萌发或停滞之后的授粉后期事件才取决于亲和性类型。花粉-柱头粘附的生化和生理解剖指出了这种相互作用的主要成分：在雄性成分中，通过脱脂消除的花粉涂层（或在相关物种拟南芥的cer突变体的情况下通过突变修饰）在粘附中起着主要作用；花粉亲本的遗传背景也很重要。在雌性方面，柱头的发育阶段和柱头薄膜的蛋白质成分对于花粉捕获至关重要。 SLG 和 SLR1 蛋白不参与花粉粘附在柱头上的初始阶段，但其中一个或两个可能参与后期阶段。
The adhesion of pollen on the stigmas of flowering plants is a critical step for the success of reproduction in angiosperms, long considered to present some specificity in terms of self-incompatibility. We carried out quantitative measurements of the pollen-stigma adhesion (expressed in Newtons) in kale (Brassica oleracea), using the flotation force of Archimedes exerted by dense sucrose solutions (50%, w/v) to release pollen grains fixed on the surface of stigmas. We demonstrate that pollen adhesion varies with the genotypes of the plants used as partners, but increases with time in all cases for about 30 to 60 min after pollination. There is no correlation with the self- or cross-status of the pollinations, nor with the self-compatible or -incompatible genotypes of the parents. Only late events of pollination, after the germination or arrest of the pollen tube, depend on compatibility type. Biochemical and physiological dissection of pollen-stigma adhesion points to major components of this interaction: among male components, the pollen coating, eliminated by delipidation (or modified by mutation in the case of the cer mutants of the related species Arabidopsis thaliana), plays a major role in adhesion; the genetic background of the pollen parent is also of some importance. On the female side, the developmental stage of the stigma and the protein constituents of the stigmatic pellicle are critical for pollen capture. The SLG and SLR1 proteins are not involved in the initial stages of pollen adhesion on the stigma but one or both may be involved in the later stages.