Epidemiology of viral respiratory tract infections in a prospective cohort of infants and toddlers attending daycare
Epidemiology of viral respiratory tract infections in a prospective cohort of infants and toddlers attending daycare
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DOI：
10.1016/j.jcv.2010.06.013
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发表时间：
2010-09-01
影响因子：
8.8
通讯作者：
Englund, Janet A.
中科院分区：
文献类型：
--
作者：
Fairchok, Mary P.;Martin, Emily T.;Englund, Janet A.
工作背景：呼吸道感染（RTIs）在日托队列的流行病学还没有探索使用分子techniques.Objectives：（1）确定RTIs的总体发病率在日托队列使用实时逆转录聚合酶链反应（RT-PCR）。(2)确定特定呼吸道病毒的相对发病率和影响，并表征和比较与这些病原体相关的临床特征。研究设计：在2006年2月至2008年4月进行的这项前瞻性队列研究中，鼻拭子从0-30个月的有症状的儿童在全日制日托。采用RT-PCR方法检测呼吸道合胞病毒（RSV）、人偏肺病毒（MPV）、流感病毒A和B、副流感病毒（PIV）、腺病毒（AdV）、人冠状病毒（CoV）和鼻病毒（RhV）。症状日记完成了每一个illustration.Results：我们跟踪了119名儿童（平均年龄10个月，范围2-24个月）为115儿童年。研究的第一年和第二年，每个儿童RTI的平均年发病率分别为4.2和1.2。在67%的RTI中至少鉴定出1种病毒。合并感染很常见（27%的RTI），RhV、CoV和AdV是最常见的合并病原体。在12%的RTI中确定了PIV，其中PIV 4的发生率较高。对我们的人口影响最大的病毒是RSV，RhV和AdV.Conclusions：使用分子技术，病毒被确定在大约两倍的RTIs以前报道的日托队列。新发现的病毒（如HMPV和CoV亚型）感染的频率和严重程度低于RSV、AdV和RhV感染。由Elsevier B出版。诉
Background: The epidemiology of respiratory tract infections (RTIs) in a daycare cohort has not been explored using molecular techniques.Objectives: (1) Determine the overall incidence of RTIs in a daycare cohort using real-time reverse transcriptase polymerase chain reaction (RT-PCR). (2) Determine the relative incidence and impact of specific respiratory viruses, and characterize and compare clinical features associated with these pathogens.Study design: In this prospective cohort study conducted from February 2006 to April 2008, nasal swabs were obtained from symptomatic children ages 0-30 months enrolled in fulltime daycare. RT-PCR was performed to detect respiratory syncytial virus (RSV), human metapneumovirus (MPV), influenza (Flu) viruses A and B, parainfluenza (PIV), adenovirus (AdV), human coronaviruses (CoV) and rhinovirus (RhV). Symptom diaries were completed for each illness.Results: We followed 119 children (mean age 10 months; range 2-24 months) for 115 child years. The mean annual incidence of RTI per child was 4.2 the first year and 1.2 the second year of the study. At least 1 virus was identified in 67% RTIs. Co-infections were common (27% RTIs), with RhV, CoV, and AdV the most common co-pathogens. PIV was identified in 12% of RTIs with a high incidence of PIV4. The viruses with the greatest impact on our population were RSV, RhV and AdV.Conclusions: Using molecular techniques, viruses were identified in approximately twice as many RTIs as previously reported in a daycare cohort. Infections with newly identified viruses, such as HMPV and CoV subtypes were less frequent and severe than infections with RSV, AdV and RhV. Published by Elsevier B. V.