Communication—Direct Detection of Methyl Salicylate Using Tri-Enzyme Based Electrochemical Sensor
Communication—Direct Detection of Methyl Salicylate Using Tri-Enzyme Based Electrochemical Sensor
复制标题
通讯——使用基于三酶的电化学传感器直接检测水杨酸甲酯
DOI：
10.1149/2.0541809jes
复制
发表时间：
2018
影响因子：
3.9
通讯作者：
Ramasamy, Ramaraja P.
中科院分区：
文献类型：
--
作者：
Fang, Yi;Zhou, Yan;Ramasamy, Ramaraja P.
描述了一种直接检测植物中生物胁迫的关键挥发性化学标记物水杨酸甲酯的新方法。该方法涉及一个4-反应级联，使用酯酶、水杨酸羟化酶和酪氨酸酶将水杨酸甲酯转化为1，2-苯醌，其中1，2-苯醌被电化学还原为儿茶酚，产生电流信号。该方法通过消除对分析物的繁琐的预处理程序的需要，极大地简化了先前描述的用于水杨酸甲酯的安培检测的程序。初步数据表明，基于三酶的检测是有前途的，比以前报道的双酶为基础的方法。
A new method for direct detection of methyl salicylate, a key volatile chemical marker for biotic stresses in plants is described. The approach involves a 4-reaction cascade that converts methyl salicylate into 1, 2-benzoquinone using esterase, salicylate hydroxylase and tyrosinase, where 1, 2-benzoquinone is electrochemically reduced to catechol resulting in an amperometric signal. The approach vastly simplifies the procedure for amperometric detection of methyl salicylate described earlier by eliminating the need for cumbersome pretreatment procedures for the analyte. Initial data suggested that the tri-enzyme based detection is promising over the previously reported bi-enzyme based methods.
使用电活性金纳米颗粒对水杨酸甲酯进行高灵敏电化学检测。
DOI：
--
发表时间：
2013
期刊：
In Analysis
影响因子：
--
作者：
Yogeswaran Umasankar;R. Ramasamy
通讯作者：
R. Ramasamy