The Detection of Spiroplasma citri by Enzyme‐linked Immunosorbent Assay (ELISA)
The Detection of Spiroplasma citri by Enzyme‐linked Immunosorbent Assay (ELISA)
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DOI：
10.1111/j.1439-0434.1978.tb03623.x
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发表时间：
1978-08
影响因子：
1.5
通讯作者：
M. F. Clark;C. Flegg;M. Bar-joseph;S. Rottem
中科院分区：
文献类型：
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作者：
M. F. Clark;C. Flegg;M. Bar-joseph;S. Rottem
该有机体很容易检测到的文化，并在新鲜收获和冻干种子收集的柑橘树顽固的疾病在1月和4月。积极的反应，叶中脉提取物，仅从7月收集的嫩叶。ELISA检测螺原体的速度和灵敏度将大大有助于现场调查和流行病学调查。Citri和其它类似的生物体，对于它们有合适的抗血清。
The organism was readily detected in cultures and in freshly harvested and freeze-dried seeds collected from citrus trees with stubborn disease in Jan. and Apr. Positive reactions with extracts of leaf mid-veins were obtained only from young leaves collected in July. The speed and sensitivity with which spiroplasma can be detected by ELISA should greatly facilitate field surveys and epidemiological investigations for S. citri and other similar organisms for which suitable antisera are available.