Expression and tissue localization of renalase, a novel soluble FAD-dependent protein, in reproductive/steroidogenic systems
Expression and tissue localization of renalase, a novel soluble FAD-dependent protein, in reproductive/steroidogenic systems
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DOI：
10.1007/s11033-012-2476-0
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发表时间：
2013-06
影响因子：
2.8
通讯作者：
Mingxue Zhou;Tong Liang;Yifeng Wang;Da Jin;Jian Wang;Liyun Jia;Shuping Zhang
中科院分区：
文献类型：
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作者：
Mingxue Zhou;Tong Liang;Yifeng Wang;Da Jin;Jian Wang;Liyun Jia;Shuping Zhang
肾酶最初在人肾中被鉴定为可溶性单胺氧化酶。在这里，我们表明肾酶主要在生殖/类固醇生成系统中表达，在卵母细胞、颗粒层、卵巢间质和黄体细胞、睾丸生精细胞和肾上腺皮质中表达特别多，表明其在生殖细胞成熟和类固醇激素调节中的功能。随着小鼠的发育，肾酶在睾丸和卵巢中的表达增加，并且其表达在怀孕小鼠的卵巢中进一步增强，表明肾酶在生殖中的活性。促性腺激素释放激素 (GnRH) 拮抗剂西曲瑞克可抑制小鼠卵巢和睾丸中的肾酶表达，表明类固醇可调节肾酶表达。瘦素是类固醇激素和生殖的效应器和调节器。令人惊讶的是，瘦素的敲除导致小鼠睾丸中肾酶表达的急剧增加。综上所述，我们的结果表明，生殖/类固醇生成系统也是肾酶分泌的来源，并且肾酶可能在生殖和激素调节中发挥关键作用。这为理解肾酶的功能提供了新的见解。
Renalase was initially identified in human kidney as a soluble monoamine oxidase. Here we show that renalase is predominantly expressed in reproductive/steroidogenic systems, with particularly substantial expression in oocytes, granulosa, interstitial and luteal cells of ovary, spermatogenic cells of testis, and cortex of adrenal gland, suggesting its function(s) in maturation of germ cells and steroid hormone regulation. Renalase expression increases in testes and ovaries as mice develop and its expression is further enhanced in the ovaries of pregnant mice, indicating an activity of renalase in reproduction. Gonadotropin-releasing hormone (GnRH) antagonist, cetrorelix, repressed renalase expression in mice ovaries and testes, suggesting that steroids regulate renalase expression. Leptin is an effector and modulator of steroid hormones and reproduction. Surprisingly, knockout of leptin causes a dramatic increase of renalase expression in mice testes. Taken together, our results suggest that reproductive/steroidogenic systems are also the sources for renalase secretion and renalase may play a critical role in reproduction and hormone regulation. This provides a novel insight into understanding the function of renalase.