Rapid genomic DNA variation in newly hybridized carp lineages derived from Cyprinus carpio (♀) x Megalobrama amblycephala (♂)
Rapid genomic DNA variation in newly hybridized carp lineages derived from Cyprinus carpio (♀) x Megalobrama amblycephala (♂)
复制标题
来自鲤鱼（雌性）x 团头鲂（雄性）的新杂交鲤鱼谱系中基因组 DNA 快速变异
DOI：
10.1186/s12863-019-0784-2
复制
发表时间：
2019-11-28
期刊：
影响因子：
2.9
通讯作者：
Liu, Shaojun
中科院分区：
文献类型：
--
作者：
Luo, Kaikun;Wang, Shi;Liu, Shaojun
背景远缘杂交可以引起表型和基因型的改变，从而导致具有遗传变异的新杂交谱系的形成。本研究利用鲤种间杂交建立了两个两性可育鲤家系，即改良二倍体鲤（IDC）家系和改良二倍体散镜鲤（IDMC）家系，（鲤鱼，2n = 100）（雌性）×钝口鲷团头鲂（Megalobrama amblycephala，2n = 48）（雄性）的遗传多样性为研究亲本及其杂交后代的遗传关系提供了良好的平台。结果研究了鲤鱼（雌）×团头鲂（雄）两种改良品系12个Hox基因的遗传变异。Hox基因簇在第一代IDC中大量存在，但在第二代中大多数不稳定遗传。与此相反，我们在第一代IDMC中没有发现明显的Hox基因突变，并且几乎所有的Hox基因簇从第一代IDMC稳定遗传到第二代IDMC。有趣的是，我们发现了明显的重组集群的Hox基因在两个改良的鲤鱼谱系，和部分重组集群的Hox基因的稳定遗传，从第一代到第二代的两种类型的改良鲤鱼谱系。另一方面，一些Hox基因逐渐成为假基因，有些基因在IDC或IDMC中完全假化。结论我们的研究结果提供了重要的证据表明，远缘杂交产生快速的基因组DNA的变化，可能会或可能不会被稳定遗传，提供了新的见解杂交的功能，在建立改进的谱系作为新的鱼类资源，用于水产养殖。
Background Distant hybridization can generate changes in phenotypes and genotypes that lead to the formation of new hybrid lineages with genetic variation. In this study, the establishment of two bisexual fertile carp lineages, including the improved diploid common carp (IDC) lineage and the improved diploid scattered mirror carp (IDMC) lineage, from the interspecific hybridization of common carp (Cyprinus carpio, 2n = 100) (female) x blunt snout bream (Megalobrama amblycephala, 2n = 48) (male), provided a good platform to investigate the genetic relationship between the parents and their hybrid progenies. Result In this study, we investigated the genetic variation of 12 Hox genes in the two types of improved carp lineages derived from common carp (female) x blunt snout bream (male). Hox gene clusters were abundant in the first generation of IDC, but most were not stably inherited in the second generation. In contrast, we did not find obvious mutations in Hox genes in the first generation of IDMC, and almost all the Hox gene clusters were stably inherited from the first generation to the second generation of IDMC. Interestingly, we found obvious recombinant clusters of Hox genes in both improved carp lineages, and partially recombinant clusters of Hox genes were stably inherited from the first generation to the second generation in both types of improved carp lineages. On the other hand, some Hox genes were gradually becoming pseudogenes, and some genes were completely pseudogenised in IDC or IDMC. Conclusions Our results provided important evidence that distant hybridization produces rapid genomic DNA changes that may or may not be stably inherited, providing novel insights into the function of hybridization in the establishment of improved lineages used as new fish resources for aquaculture.