Stem Cell Signaling in Arabidopsis Requires CRN to Localize CLV2 to the Plasma Membrane
Stem Cell Signaling in Arabidopsis Requires CRN to Localize CLV2 to the Plasma Membrane
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DOI：
10.1104/pp.109.149930
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发表时间：
2010-01-01
期刊：
影响因子：
7.4
通讯作者：
Simon, Ruediger
中科院分区：
文献类型：
--
作者：
Bleckmann, Andrea;Weidtkamp-Peters, Stefanie;Simon, Ruediger
拟南芥（Arabidopsis thaliana）茎和花分生组织中的干细胞数量受CLAVATA 3（CLV 3）信号通路调节。CLV 3肽的感知需要受体激酶CLV 1、受体样蛋白CLV 2和激酶CORYNE（CRN）。遗传分析表明，CLV 2和CRN共同作用，并与CLV 1平行。我们研究了荧光蛋白标签的受体融合体的细胞内定位及其通过荧光共振能量转移效率相互作用的能力。我们发现CLV 2和CRN需要彼此才能从内质网输出并定位到质膜（PM）。CRN容易形成同聚体并通过跨膜结构域和相邻的跨膜序列与CLV 2相互作用。CLV 1在PM处独立于CLV 2和CRN形成同聚体。我们建议，CLV 3信号被感知的四聚体CLV 2/CRN复合物和CLV 1同源二聚体，本地化的PM，并可以通过CRN相互作用。
Stem cell number in shoot and floral meristems of Arabidopsis (Arabidopsis thaliana) is regulated by the CLAVATA3 (CLV3) signaling pathway. Perception of the CLV3 peptide requires the receptor kinase CLV1, the receptor-like protein CLV2, and the kinase CORYNE (CRN). Genetic analysis suggested that CLV2 and CRN act together and in parallel with CLV1. We studied the intracellular localization of receptor fusions with fluorescent protein tags and their capacities for interaction via efficiency of fluorescence resonance energy transfer. We found that CLV2 and CRN require each other for export from the endoplasmic reticulum and localization to the plasma membrane (PM). CRN readily forms homomers and interacts with CLV2 through the transmembrane domain and adjacent juxtamembrane sequences. CLV1 forms homomers independently of CLV2 and CRN at the PM. We propose that the CLV3 signal is perceived by a tetrameric CLV2/CRN complex and a CLV1 homodimer that localize to the PM and can interact via CRN.