Use of tetramethylbenzidine in plasma hemoglobin assay.

Use of tetramethylbenzidine in plasma hemoglobin assay.
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四甲基联苯胺在血浆血红蛋白测定中的应用。

DOI:
10.1093/clinchem/23.4.749
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发表时间:
1977
期刊:
影响因子:
9.3
通讯作者:
D. Vanderjagt
D. Vanderjagt
中科院分区:
医学1区
文献类型:
--
作者:
J. Standefer;D. Vanderjagt

文献摘要

被引文献

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用3,3',5,5'-四甲基联苯胺代替联苯胺(致癌物质)作为显色还原物质,可以测定血浆血红蛋白。灵敏度、精密度和准确度与使用联苯胺的方法相当。可以通过用过氧化氢预孵育来调节血浆抑制。
Plasma hemoglobin can be assayed by substituting 3,3',5,5'-tetramethylbenzidine for benzidine (a carcinogen) as the chromogenic reducing substance. Sensitivity, precision, and accuracy compare well with methods in which benzidine is used. Plasma inhibition can be accommodated by preincubation with hydrogen peroxide.