Rapidity of compensatory lung growth following pneumonectomy in adult rats.
Rapidity of compensatory lung growth following pneumonectomy in adult rats.
复制标题
成年大鼠肺切除术后代偿性肺生长的快速性。
DOI：
10.1152/jappl.1979.46.2.326
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
C. A. Watkins
中科院分区：
文献类型：
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作者：
D. Rannels;D. M. White;C. A. Watkins
研究人员用大鼠（330 g）研究了肺切除术后肺生长的速度，其中肺重量与体重比和肺细胞大小已经稳定。左肺切除后，右肺重量在7天内由823 mg增加到1.161 mg。假手术动物右肺重量无明显变化。第7天，全肺切除大鼠右肺重量体重比与假手术大鼠双肺重量体重比相等；这些数值在第14天保持不变。右肺各肺叶的生长一般与其初始重量成正比。与未手术的对照组相比，假手术或全肺切除的动物肺的干湿重量比没有变化。第2天，右肺总RNA和蛋白质显著增加，第7天达到与假手术动物双肺相同的水平。在原位灌注120分钟时，肺蛋白的合成在第3天升高25%。[3H]胸苷在DNA中的掺入在第2天有所增加，在第3天增加了4倍，与肺内总DNA的初始积累相对应。这些观察结果表明，细胞大小的增加可能伴随着肺切除术后早期代偿性生长，但反应的主要部分涉及细胞增生。
The rapidity with which lung growth was initiated following pneumonectomy was investigated using rats (330 g) in which lung weight-to-body weight ratio and lung cell size had stabilized. Following removal of the left lung, right lung weight increased from 823 to 1.161 mg within 7 days. Right lung weight in sham-operated animals did not change significantly. At day 7, right lung weight-to-body weight ratio in pneumonectomized rats was equal to that of both lungs in sham-operated animals; these values remained equal through day 14. Growth of individual lobes of the right lung was generally in proportion to their initial weights. Dry-to-wet weight ratio in either lung of sham-operated or pneumonectomized animals was unchanged, as compared to unoperated controls. Total right lung RNA and protein increased significantly by day 2 and reached levels equal to those in both lungs of sham-operated animals by day 7. Synthesis of lung proteins, estimated during 120 min of perfusion in situ, was elevated 25% on day 3. Incorporation of [3H]thymidine into DNA increased somewhat on day 2 and was elevated fourfold on day 3, corresponding with the initial accumulation of total DNA within the lung. These observations suggested that increased cell size may accompany early compensatory growth following pneumonectomy, but that the major portion of the response involved cellular hyperplasia.