Effects of neonatal asphyxia on the serotonin neuron system in the developing brain studied by immunohistochemistry.
Effects of neonatal asphyxia on the serotonin neuron system in the developing brain studied by immunohistochemistry.
复制标题
通过免疫组织化学研究新生儿窒息对发育中大脑中血清素神经元系统的影响。
DOI：
10.1159/000111688
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发表时间：
1992
影响因子：
2.9
通讯作者：
T. Sawada
中科院分区：
文献类型：
--
作者：
Y. Takeuchi;K. Fujiwara;K. Ishimura;S. Aoki;S. Okano;I. Yamazoe;H. Yoshioka;T. Sawada
用免疫组织化学方法检测新生儿窒息对5-羟色胺神经系统的影响。2日龄雄性小鼠暴露于完全窒息(100%CO2)30min。自发存活的小鼠在15天、30天和60天时分别经心脏注入固定剂。60日龄免疫组织化学定量分析显示，中缝背核、中缝桥核、锥体下区、中缝全系统及全脑内5-羟色胺免疫反应阳性细胞体数量显著减少，而中缝尾侧系统5-羟色胺免疫阳性细胞体数量无明显减少。据推测，15天龄全窒息小鼠脑内不同部位的5-羟色胺免疫反应纤维均有退行性变化，30天和60天龄时，除脑干尾侧部分外，几乎所有部位的退行性纤维数量均减少。这些结果表明，新生儿窒息引起5-羟色胺神经元系统的永久性改变，并存在地区差异。
The effects of neonatal asphyxia on the serotonin neuron system were examined using the immunoperoxidase method. Male mice, 2 days of age, were exposed to total asphyxia (100% CO2) for 30 min. Mice that spontaneously survived were perfused transcardially with a fixative at 15, 30 and 60 days of age. Quantitative immunohistochemical analysis at 60 days of age demonstrated a significant decrease in the numbers of serotonin-immunoreactive cell bodies in the nucleus raphe dorsalis, the nucleus raphe pontis, the subpyramidal region, the total raphe system and the whole brain, while no significant reduction in the number of serotonin-immunoreactive cell bodies was observed in the caudal raphe system. Presumably degenerative changes in serotonin-immunoreactive fibers were observed in various parts of the brain of mice subjected to total asphyxia at 15 days of age, and the numbers of degenerated fibers decreased in almost all parts of the brain, the exception being the caudal portion of the brainstem, at 30 and 60 days of age. These results suggested that neonatal asphyxia induced permanent changes in the serotonin neuron system, with regional differences.