Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique.
Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique.
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DOI：
10.1016/j.ijantimicag.2017.02.013
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发表时间：
2017-07
影响因子：
10.8
通讯作者：
J. Pei;Lei Jiang
中科院分区：
文献类型：
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作者：
J. Pei;Lei Jiang
中国大鲵Andrias davidianus已被用于中药数十年。然而，没有抗菌肽（AMP）已被描述从A。davidianus直到现在。在这里，我们描述了一种新的AMP（andricin 01）分离自A。大卫。使用创新的磁性细胞膜分离技术回收肽，并使用质谱法和圆二色性（CD）光谱进行了表征。Andricin 01由10个氨基酸残基组成，总分子量为955.1 Da。CD光谱分析得到的结果与原型无规卷曲光谱相似，与当前生物信息学工具计算的三维渲染一致。Andricin 01对革兰氏阴性菌和革兰氏阳性菌均有抑制作用。此外，在最小细菌浓度下的肽没有显示出对人肝细胞或肾细胞的细胞毒性，并且没有显示出对红细胞的溶血活性，表明其对于人类使用是潜在安全和有效的。Andricin 01显示出作为一种新型抗菌剂的前景，可能会为新药的开发提供一种见解。
Andrias davidianus, the Chinese giant salamander, has been used in traditional Chinese medicine for many decades. However, no antimicrobial peptides (AMPs) have been described fromA. davidianusuntil now. Here we describe a novel AMP (andricin 01) isolated from the mucus ofA. davidianus. The peptide was recovered using an innovative magnetic cell membrane separation technique and was characterised using mass spectrometry and circular dichroism (CD) spectroscopy. Andricin 01 is comprised of ten amino acid residues with a total molecular mass of 955.1 Da. CD spectrum analysis gave results similar to the archetypal random coil spectrum, consistent with the three-dimensional rendering calculated by current bioinformatics tools. Andricin 01 was found to be inhibitory both to Gram-negative and Gram-positive bacteria. Furthermore, the peptide at the minimal bacterial concentration did not show cell cytotoxicity against human hepatocytes or renal cells and did not show haemolytic activity against red blood cells, indicating that is potentially safe and effective for human use. Andricin 01 shows promise as a novel antibacterial that may provide an insight into the development of new drugs.