Dimensions of the Great Intrathoracic Arteries of Early Mouse Fetuses of the C57BL/6 Strain
Dimensions of the Great Intrathoracic Arteries of Early Mouse Fetuses of the C57BL/6 Strain
复制标题
C57BL/6品系早期小鼠胎儿胸内大动脉的尺寸
DOI：
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
W. Weninger
中科院分区：
文献类型：
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作者：
S. Geyer;B. Maurer;Kathrin Dirnbacher;W. Weninger
仔细分析畸形小鼠胎儿的形态表型是研究心血管畸形起源的重要步骤。动脉狭窄和扩张的检测需要度量分析和定义胎儿动脉正常尺寸的参考数据。由于近交系的遗传背景强烈影响动脉的尺寸，因此参考数据是特定于品系的。我们的目的是创建客观的参考数据，用于诊断 C57BL/6 背景下培育的早期小鼠胎儿胸内大动脉重要区段的狭窄和扩张。采用高分辨率内窥镜 (HREM) 技术，我们根据 Theiler (TS22) 的标准，创建了 30 个发育阶段 22 的小鼠胎儿胸内大动脉的体数据和三维 (3D) 计算机模型。然后，我们沿着升主动脉、肺干、动脉导管和降主动脉定义了可重复的测量位置，并使用最近发布的协议来测量这些位置处动脉管腔的直径。我们给出以毫米为单位测量的直径所获得的值。我们还提出了每条血管测量的直径与升主动脉和肺干管腔直径的关系。我们提供的测量值和相对值客观地定义了正常发育的 C57BL/6 品系 TS22 小鼠胎儿胸内大动脉重要节段的尺寸。它们将作为诊断畸形胎儿中测量节段的狭窄和扩张的可靠参考数据。
Careful analysis of the morphological phenotype of malformed mouse fetuses is an essential step in researching the genesis of cardiovascular malformations. The detection of arterial stenosis and dilation requires metric analysis and reference data defining the normal dimensions of the fetal arteries. Since the genetic background of inbred strains strongly influences the dimensions of the arteries, reference data are strain specific. We aimed at creating objective reference data for diagnosing the stenosis and dilation of important segments of the great intrathoracic arteries of early mouse fetuses bred on the C57BL/6 background. Employing the high-resolution episcopic microscopy (HREM) technique, we created volume data and three-dimensional (3D) computer models of the great intrathoracic arteries of 30 mouse fetuses of developmental stage 22 according to Theiler (TS22). We then defined reproducible measurement positions along the ascending aorta, the pulmonary trunk, the ductus arteriosus and the descending aorta and used a recently published protocol to measure the diameter of the lumen of the arteries at these positions. We present the values obtained for the diameters as measured inm. We also present the relation of the diameter measured for each blood vessel and the diameters of the lumen of the ascending aorta and pulmonary trunk. The measurements and relative values we provide objectively define the dimensions of important segments of the great intrathoracic arteries of normally developed TS22 mouse fetuses of the C57BL/6 strain. They will function as reliable reference data for diagnosing stenosis and dilation of the measured segments in malformed fetuses.