RECOMBINANT VACCINIA VIRUS EXPRESSING THE PR/8 INFLUENZA HEMAGGLUTININ GENE OVERCOMES THE IMPAIRED IMMUNE-RESPONSE AND INCREASED SUSCEPTIBILITY OF OLD MICE TO INFLUENZA INFECTION
RECOMBINANT VACCINIA VIRUS EXPRESSING THE PR/8 INFLUENZA HEMAGGLUTININ GENE OVERCOMES THE IMPAIRED IMMUNE-RESPONSE AND INCREASED SUSCEPTIBILITY OF OLD MICE TO INFLUENZA INFECTION
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DOI：
10.1093/infdis/168.2.352
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发表时间：
1993-08-01
影响因子：
6.4
通讯作者：
WEKSLER, ME
中科院分区：
文献类型：
--
作者：
BENYEHUDA, A;EHLEITER, D;WEKSLER, ME
尽管免疫接种，老年人在病毒性流感后的发病率和死亡率仍有所增加。使用流感感染的小鼠模型来寻找更有效的诱导流感免疫的方法。尽管先前已免疫接种流感病毒，但经PR/8流感病毒鼻内激发后，老年（18个月）BALB/c小鼠比年轻（2个月）BALB/c小鼠更易患流感肺炎。老年小鼠对活流感病毒攻击的抵抗力下降与抗血凝素抗体和细胞毒性T淋巴细胞的产生受损有关。相反，用表达PR/8流感血凝素基因的重组牛痘病毒免疫老年小鼠，保护它们免受活流感病毒的鼻内攻击，并产生高水平的抗PR/8流感病毒血凝素抗体和PR/8特异性细胞毒性T细胞。重组疫苗克服了常规病毒疫苗接种后的年龄相关免疫缺陷。
Elderly humans have increased morbidity and mortality after viral influenza despite immunization. A mouse model of influenza infection was used to search for a more effective way to induce immunity to influenza. Old (18 months) BALB/c mice were more susceptible to influenza pneumonia than young (2 months) BALB/c mice after intranasal challenge with PR/8 influenza virus despite prior immunization with influenza virus. The decreased resistance to live influenza virus challenge was associated with an impaired generation of anti-hemagglutinin antibody and cytotoxic T lymphocytes in old mice. In contrast, immunization of old mice with a recombinant vaccinia virus expressing the PR/8 influenza hemagglutinin gene protected them from intranasal challenge with live influenza virus and generated high levels of anti-PR/8 influenza virus hemagglutinin antibody and PR/8-specific cytotoxic T cells. Recombinant vaccine overcame the age-associated immune defect that follows the administration of conventional viral vaccine.